Part 1 Alignment with totipotency datasets
Moyra Lawrence
2023-10-19
Loading libraries
dyn.load("/home/moyra.lawrence/miniconda3/envs/monocle3/lib/libgeos_c.so.1")
library(Seurat)## Warning in fun(libname, pkgname): rgeos: versions of GEOS runtime 3.11.0-CAPI-1.17.0
## and GEOS at installation 3.8.0-CAPI-1.13.1differlibrary(patchwork)
library(ggplot2)
library(gridExtra)
library(openxlsx)
library(svglite)
library(dplyr)
library(gt)
library(tidyverse)
library(stringi)
library(SeuratObject)
library(biomaRt)
library(stringr)#Loading Hu et al., 2023
Hu <- Read10X(data.dir = '/home/moyra.lawrence/R_analysis/Comparison/Hu')
Hu = CreateSeuratObject(counts = Hu, project = "Hu")Checking filtering
Hu[["percent.mt"]] <- PercentageFeatureSet(Hu, pattern = "^mt-")
VlnPlot(Hu, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)
VlnPlot(Hu, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
FeatureScatter(Hu, feature1 = "nCount_RNA", feature2 = "percent.mt")
FeatureScatter(Hu, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
##Filtering
1000 < nFeature_RNA
2000 < nCount_RNA <100,000
0 =< percent.mt < 10
Hu.filtered <- subset(Hu, subset = nFeature_RNA > 1000 & nCount_RNA > 2000 & nCount_RNA < 100000 & percent.mt < 10)
Hu.filtered## An object of class Seurat
## 32285 features across 9795 samples within 1 assay
## Active assay: RNA (32285 features, 0 variable features)Confirming filtering
vp1 <- VlnPlot(Hu.filtered, pt.size=0, features = c("nFeature_RNA")) + theme(legend.position = 'none')
vp2 <- VlnPlot(Hu.filtered, pt.size=0, features = c("nCount_RNA")) + theme(legend.position = 'none')
vp3 <- VlnPlot(Hu.filtered, pt.size=0, features = c("percent.mt")) + theme(legend.position = 'none')
vp1.log <- VlnPlot(Hu.filtered, pt.size=0, features = c("nFeature_RNA"),log=TRUE)
vp2.log <- VlnPlot(Hu.filtered, pt.size=0, features = c("nCount_RNA"),log=TRUE)
vp3.log <- VlnPlot(Hu.filtered, pt.size=0, features = c("percent.mt"),log=TRUE) grid.arrange(vp1,vp2,vp3, nrow=1)
grid.arrange(vp1.log,vp2.log,vp3.log, nrow=1)
FeatureScatter(Hu.filtered, "nCount_RNA", "percent.mt", pt.size=0.5) + scale_y_continuous(limits=c(0,100)) + scale_x_continuous(limits=c(0,100000))
FeatureScatter(Hu.filtered, "nCount_RNA", "nFeature_RNA", pt.size=0.5) + scale_y_continuous(limits=c(0,12000)) + scale_x_continuous(limits=c(0,100000))
#Loading Xu et al., 2022
XuESC1 <- Read10X(data.dir = '/home/moyra.lawrence/R_analysis/Comparison/Xu/Xu_ES1/ES1')
XuESC2 <- Read10X(data.dir = '/home/moyra.lawrence/R_analysis/Comparison/Xu/Xu_ES2/ES2')
XuEPS1 <- Read10X(data.dir = '/home/moyra.lawrence/R_analysis/Comparison/Xu/Xu_EPS1/EPS1')
XuEPS2 <- Read10X(data.dir = '/home/moyra.lawrence/R_analysis/Comparison/Xu/Xu_EPS2/EPS2')
XuEPSTPS <- Read10X(data.dir = '/home/moyra.lawrence/R_analysis/Comparison/Xu/Xu_EPS_TPS/EPS-TPS2')
Xu2CTPS <- Read10X(data.dir = '/home/moyra.lawrence/R_analysis/Comparison/Xu/Xu_2C_TPS/2C-TPS1')
XuESC1 = CreateSeuratObject(counts = XuESC1, project = "XuESC")
XuESC2 = CreateSeuratObject(counts = XuESC2, project = "XuESC")
XuEPS1 = CreateSeuratObject(counts = XuEPS1, project = "XuEPSC")
XuEPS2 = CreateSeuratObject(counts = XuEPS2, project = "XuEPSC")
XuEPSTPS = CreateSeuratObject(counts = XuEPSTPS, project = "XuEPSTPS")
Xu2CTPS = CreateSeuratObject(counts = Xu2CTPS, project = "Xu2CTPS")Quality plot XuESC1
XuESC1[["percent.mt"]] <- PercentageFeatureSet(XuESC1, pattern = "^mt-")
VlnPlot(XuESC1, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)
VlnPlot(XuESC1, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
FeatureScatter(XuESC1, feature1 = "nCount_RNA", feature2 = "percent.mt")
FeatureScatter(XuESC1, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
### Quality plot XuESC2
XuESC2[["percent.mt"]] <- PercentageFeatureSet(XuESC2, pattern = "^mt-")
VlnPlot(XuESC2, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)
VlnPlot(XuESC2, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
FeatureScatter(XuESC2, feature1 = "nCount_RNA", feature2 = "percent.mt")
FeatureScatter(XuESC2, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
### Quality plot XuEPS1
XuEPS1[["percent.mt"]] <- PercentageFeatureSet(XuEPS1, pattern = "^mt-")
VlnPlot(XuEPS1, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)
VlnPlot(XuEPS1, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
FeatureScatter(XuEPS1, feature1 = "nCount_RNA", feature2 = "percent.mt")
FeatureScatter(XuEPS1, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
### Quality plot XuEPS2
XuEPS2[["percent.mt"]] <- PercentageFeatureSet(XuEPS2, pattern = "^mt-")
VlnPlot(XuEPS2, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)
VlnPlot(XuEPS2, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
FeatureScatter(XuEPS2, feature1 = "nCount_RNA", feature2 = "percent.mt")
FeatureScatter(XuEPS2, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
### Quality plot XuEPSTPS
XuEPSTPS[["percent.mt"]] <- PercentageFeatureSet(XuEPSTPS, pattern = "^mt-")
VlnPlot(XuEPSTPS, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)
VlnPlot(XuEPSTPS, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
FeatureScatter(XuEPSTPS, feature1 = "nCount_RNA", feature2 = "percent.mt")
FeatureScatter(XuEPSTPS, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
Quality plot Xu2CTPS
Xu2CTPS[["percent.mt"]] <- PercentageFeatureSet(Xu2CTPS, pattern = "^mt-")
VlnPlot(Xu2CTPS, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)
VlnPlot(Xu2CTPS, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
FeatureScatter(Xu2CTPS, feature1 = "nCount_RNA", feature2 = "percent.mt")
FeatureScatter(Xu2CTPS, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
##Filtering Xu
2000 < nFeature_RNA
4000 < nCount_RNA <100,000
0 =< percent.mt < 10 (12 for EPS2, 15 for 2CTPS)
XuESC1.filtered <- subset(XuESC1, subset = nFeature_RNA > 2000 & nCount_RNA > 4000 & nCount_RNA < 100000 & percent.mt < 10)
XuESC2.filtered <- subset(XuESC2, subset = nFeature_RNA > 2000 & nCount_RNA > 4000 & nCount_RNA < 100000 & percent.mt < 10)
XuEPS1.filtered <- subset(XuEPS1, subset = nFeature_RNA > 2000 & nCount_RNA > 4000 & nCount_RNA < 100000 & percent.mt < 10)
XuEPS2.filtered <- subset(XuEPS2, subset = nFeature_RNA > 2000 & nCount_RNA > 4000 & nCount_RNA < 100000 & percent.mt < 10)
XuEPSTPS.filtered <- subset(XuEPSTPS, subset = nFeature_RNA > 2000 & nCount_RNA > 4000 & nCount_RNA < 100000 & percent.mt < 12)
Xu2CTPS.filtered <- subset(Xu2CTPS, subset = nFeature_RNA > 2000 & nCount_RNA > 4000 & nCount_RNA < 100000 & percent.mt < 15)Confirming filtering
VlnPlot(XuESC1.filtered, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
VlnPlot(XuESC2.filtered, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
VlnPlot(XuEPS1.filtered, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
VlnPlot(XuEPS2.filtered, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
VlnPlot(XuEPSTPS.filtered, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
VlnPlot(Xu2CTPS.filtered, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
#Cossec
countsData <- read.delim(file="/home/moyra.lawrence/R_analysis/Comparison/Cossec/GSE180679_counts_run1.csv.gz", header=TRUE, sep=",", row.names=1)
metaData <- read.delim(file="/home/moyra.lawrence/R_analysis/Comparison/Cossec/GSE180679_meta_run1.csv.gz", header=TRUE, sep=",", row.names=1)
gene2 <- rownames(countsData)
gene3 <- str_replace(gene2,
pattern = ".[0-9]+$",
replacement = "")
ensembl = useMart(biomart = "ensembl", dataset="mmusculus_gene_ensembl")
ens2 = getBM(attributes=c('ensembl_gene_id', 'mgi_symbol'),
filters = 'ensembl_gene_id',
values = gene3,
mart = ensembl)
countsData$Gene <- gene3
merge2 <- merge(countsData, ens2, by.x="Gene", by.y = "ensembl_gene_id")
merge2["mgi_symbol"][merge2["mgi_symbol"] == ''] <- "Unknown"
names2 <- make.unique(merge2$mgi_symbol)
names2 <- gsub("_", "-", names2)
names2 <- gsub("\\.", "-", names2)
names2 <- make.unique(names2)
rownames(merge2) <- names2
merge2 <- merge2[,-1]
merge2 <- merge2[,-5377]
Cossec <- CreateSeuratObject(counts=merge2, project="Cossec", meta.data=metaData)
Cossec$sample <- NULL
Cossec$index <- NULL
Cossec$well_coordinates<- NULL
Cossec$FSC.A<- NULL
Cossec$FSC.W<- NULL
Cossec$FSC.H<- NULL
Cossec$SSC.A<- NULL
Cossec$SSC.W<- NULL
Cossec$SSC.H<- NULL
Cossec$PE.A<- NULL
Cossec$PE.Texas.Red.A<- NULL
Cossec$PE.Cy5.A<- NULL
Cossec$PE.Cy5.5.A<- NULL
Cossec$PE.Cy7.A<- NULL
Cossec$APC.A<- NULL
Cossec$Alexa.Fluor.700.A<- NULL
Cossec$APC.Cy7.A <- NULL
Cossec$FITC.A <- NULL
Cossec$PerCP.Cy5.5.A <- NULL
Cossec$BV421.A <- NULL
Cossec$AmCyan.A <- NULL
Cossec$BV605.A <- NULL
Cossec$BV650.A <- NULL
Cossec$BV711.A <- NULL
Cossec$BV786.A <- NULL
Cossec$Time <- NULL
Cossec$XLoc <- NULL
Cossec$YLoc <- NULL
Cossec$orig.ident <- "Cossec"Quality plot Cossec
Cossec[["percent.mt"]] <- PercentageFeatureSet(Cossec, pattern = "^mt-")
VlnPlot(Cossec, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)
VlnPlot(Cossec, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
FeatureScatter(Cossec, feature1 = "nCount_RNA", feature2 = "percent.mt")
FeatureScatter(Cossec, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
##Filtering Cossec
2000 < nFeature_RNA
2000 < nCount_RNA <100,000
Cossec.filtered <- subset(Cossec, subset = nFeature_RNA > 2000 & nCount_RNA > 2000 & nCount_RNA < 100000)
Cossec.filtered## An object of class Seurat
## 52776 features across 2458 samples within 1 assay
## Active assay: RNA (52776 features, 0 variable features)Confirming filtering
vp1 <- VlnPlot(Cossec.filtered, pt.size=0, features = c("nFeature_RNA")) + theme(legend.position = 'none')
vp2 <- VlnPlot(Cossec.filtered, pt.size=0, features = c("nCount_RNA")) + theme(legend.position = 'none')
vp1.log <- VlnPlot(Cossec.filtered, pt.size=0, features = c("nFeature_RNA"),log=TRUE)
vp2.log <- VlnPlot(Cossec.filtered, pt.size=0, features = c("nCount_RNA"),log=TRUE)grid.arrange(vp1,vp2, nrow=1)
grid.arrange(vp1.log,vp2.log, nrow=1)
FeatureScatter(Cossec.filtered, "nCount_RNA", "nFeature_RNA", pt.size=0.5) + scale_y_continuous(limits=c(0,12000)) + scale_x_continuous(limits=c(0,100000))
#Yu
#listAttributes(ensembl, page="feature_page")
YuC <- data.table::fread("/home/moyra.lawrence/R_analysis/Comparison/Yu/GSE166027_single_cell_gene_expression_Control(1).csv.gz", data.table = FALSE)
names(YuC)[2] <- 'Gene'
YuC <- YuC[,-1]
YuC <- YuC[,-2]
YuC <- YuC[,-2]
YuC <- YuC[,-2]
gene2 <- YuC$Gene
gene3 <- str_replace(gene2,
pattern = ".[0-9]+$",
replacement = "")
ensembl = useMart(biomart = "ensembl", dataset="mmusculus_gene_ensembl")
ens2 = getBM(attributes=c('ensembl_gene_id', 'mgi_symbol'),
filters = 'ensembl_gene_id',
values = gene3,
mart = ensembl)
YuC$Gene <- gene3
merge2 <- merge(YuC, ens2, by.x="Gene", by.y = "ensembl_gene_id")
merge2["mgi_symbol"][merge2["mgi_symbol"] == ''] <- "Unknown"
names2 <- make.unique(merge2$mgi_symbol)
names2 <- gsub("_", "-", names2)
names2 <- gsub("\\.", "-", names2)
names2 <- make.unique(names2)
rownames(merge2) <- names2
merge2 <- merge2[,-1]
merge2 <- merge2[,-3839]
YuC <- CreateSeuratObject(counts=merge2, project="YuC")
YuC$orig.ident## bc0001 bc0002 bc0003 bc0004 bc0005 bc0006 bc0007 bc0008 bc0009 bc0010 bc0011
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0012 bc0013 bc0014 bc0015 bc0016 bc0017 bc0018 bc0019 bc0020 bc0021 bc0022
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0023 bc0024 bc0025 bc0026 bc0027 bc0028 bc0029 bc0030 bc0031 bc0032 bc0033
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0034 bc0035 bc0036 bc0037 bc0038 bc0039 bc0040 bc0041 bc0042 bc0043 bc0044
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0045 bc0046 bc0047 bc0048 bc0049 bc0050 bc0051 bc0052 bc0053 bc0054 bc0055
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0056 bc0057 bc0058 bc0059 bc0060 bc0061 bc0062 bc0063 bc0064 bc0065 bc0066
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0067 bc0068 bc0069 bc0070 bc0071 bc0072 bc0073 bc0074 bc0075 bc0076 bc0077
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0078 bc0079 bc0080 bc0081 bc0082 bc0083 bc0084 bc0085 bc0086 bc0087 bc0088
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0089 bc0090 bc0091 bc0092 bc0093 bc0094 bc0095 bc0096 bc0097 bc0098 bc0099
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0100 bc0101 bc0102 bc0103 bc0104 bc0105 bc0106 bc0107 bc0108 bc0109 bc0110
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0111 bc0112 bc0113 bc0114 bc0115 bc0116 bc0117 bc0118 bc0119 bc0120 bc0121
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0122 bc0123 bc0124 bc0125 bc0126 bc0127 bc0128 bc0129 bc0130 bc0131 bc0132
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0133 bc0134 bc0135 bc0136 bc0137 bc0138 bc0139 bc0140 bc0141 bc0142 bc0143
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0144 bc0145 bc0146 bc0147 bc0148 bc0149 bc0150 bc0151 bc0152 bc0153 bc0154
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0155 bc0156 bc0157 bc0158 bc0159 bc0160 bc0161 bc0162 bc0163 bc0164 bc0165
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0166 bc0167 bc0168 bc0169 bc0170 bc0171 bc0172 bc0173 bc0174 bc0175 bc0176
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0177 bc0178 bc0179 bc0180 bc0181 bc0182 bc0183 bc0184 bc0185 bc0186 bc0187
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0188 bc0189 bc0190 bc0191 bc0192 bc0193 bc0194 bc0195 bc0196 bc0197 bc0198
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0199 bc0200 bc0201 bc0202 bc0203 bc0204 bc0205 bc0206 bc0207 bc0208 bc0209
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0210 bc0211 bc0212 bc0213 bc0214 bc0215 bc0216 bc0217 bc0218 bc0219 bc0220
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0221 bc0222 bc0223 bc0224 bc0225 bc0226 bc0227 bc0228 bc0229 bc0230 bc0231
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0232 bc0233 bc0234 bc0235 bc0236 bc0237 bc0238 bc0239 bc0240 bc0241 bc0242
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0243 bc0244 bc0245 bc0246 bc0247 bc0248 bc0249 bc0250 bc0251 bc0252 bc0253
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0254 bc0255 bc0256 bc0257 bc0258 bc0259 bc0260 bc0261 bc0262 bc0263 bc0264
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0265 bc0266 bc0267 bc0268 bc0269 bc0270 bc0271 bc0272 bc0273 bc0274 bc0275
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0276 bc0277 bc0278 bc0279 bc0280 bc0281 bc0282 bc0283 bc0284 bc0285 bc0286
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0287 bc0288 bc0289 bc0290 bc0291 bc0292 bc0293 bc0294 bc0295 bc0296 bc0297
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0298 bc0299 bc0300 bc0301 bc0302 bc0303 bc0304 bc0305 bc0306 bc0307 bc0308
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0309 bc0310 bc0311 bc0312 bc0313 bc0314 bc0315 bc0316 bc0317 bc0318 bc0319
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0320 bc0321 bc0322 bc0323 bc0324 bc0325 bc0326 bc0327 bc0328 bc0329 bc0330
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0331 bc0332 bc0333 bc0334 bc0335 bc0336 bc0337 bc0338 bc0339 bc0340 bc0341
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0342 bc0343 bc0344 bc0345 bc0346 bc0347 bc0348 bc0349 bc0350 bc0351 bc0352
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0353 bc0354 bc0355 bc0356 bc0357 bc0358 bc0359 bc0360 bc0361 bc0362 bc0363
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0364 bc0365 bc0366 bc0367 bc0368 bc0369 bc0370 bc0371 bc0372 bc0373 bc0374
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0375 bc0376 bc0377 bc0378 bc0379 bc0380 bc0381 bc0382 bc0383 bc0384 bc0385
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0386 bc0387 bc0388 bc0389 bc0390 bc0391 bc0392 bc0393 bc0394 bc0395 bc0396
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0397 bc0398 bc0399 bc0400 bc0401 bc0402 bc0403 bc0404 bc0405 bc0406 bc0407
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0408 bc0409 bc0410 bc0411 bc0412 bc0413 bc0414 bc0415 bc0416 bc0417 bc0418
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0419 bc0420 bc0421 bc0422 bc0423 bc0424 bc0425 bc0426 bc0427 bc0428 bc0429
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0430 bc0431 bc0432 bc0433 bc0434 bc0435 bc0436 bc0437 bc0438 bc0439 bc0440
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0441 bc0442 bc0443 bc0444 bc0445 bc0446 bc0447 bc0448 bc0449 bc0450 bc0451
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0452 bc0453 bc0454 bc0455 bc0456 bc0457 bc0458 bc0459 bc0460 bc0461 bc0462
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0463 bc0464 bc0465 bc0466 bc0467 bc0468 bc0469 bc0470 bc0471 bc0472 bc0473
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0474 bc0475 bc0476 bc0477 bc0478 bc0479 bc0480 bc0481 bc0482 bc0483 bc0484
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0485 bc0486 bc0487 bc0488 bc0489 bc0490 bc0491 bc0492 bc0493 bc0494 bc0495
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0496 bc0497 bc0498 bc0499 bc0500 bc0501 bc0502 bc0503 bc0504 bc0505 bc0506
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0507 bc0508 bc0509 bc0510 bc0511 bc0512 bc0513 bc0514 bc0515 bc0516 bc0517
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0518 bc0519 bc0520 bc0521 bc0522 bc0523 bc0524 bc0525 bc0526 bc0527 bc0528
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0529 bc0530 bc0531 bc0532 bc0533 bc0534 bc0535 bc0536 bc0537 bc0538 bc0539
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0540 bc0541 bc0542 bc0543 bc0544 bc0545 bc0546 bc0547 bc0548 bc0549 bc0550
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0551 bc0552 bc0553 bc0554 bc0555 bc0556 bc0557 bc0558 bc0559 bc0560 bc0561
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0562 bc0563 bc0564 bc0565 bc0566 bc0567 bc0568 bc0569 bc0570 bc0571 bc0572
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0573 bc0574 bc0575 bc0576 bc0577 bc0578 bc0579 bc0580 bc0581 bc0582 bc0583
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0584 bc0585 bc0586 bc0587 bc0588 bc0589 bc0590 bc0591 bc0592 bc0593 bc0594
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc0595 bc0596 bc0597 bc0598 bc0599 bc0600 bc0601 bc0602 bc0603 bc0604 bc0605
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## bc1024 bc1025 bc1026 bc1027 bc1028 bc1029 bc1030 bc1031 bc1032 bc1033 bc1034
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## bc1035 bc1036 bc1037 bc1038 bc1039 bc1040 bc1041 bc1042 bc1043 bc1044 bc1045
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## bc1046 bc1047 bc1048 bc1049 bc1050 bc1051 bc1052 bc1053 bc1054 bc1055 bc1056
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## bc1057 bc1058 bc1059 bc1060 bc1061 bc1062 bc1063 bc1064 bc1065 bc1066 bc1067
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## bc1068 bc1069 bc1070 bc1071 bc1072 bc1073 bc1074 bc1075 bc1076 bc1077 bc1078
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## bc1079 bc1080 bc1081 bc1082 bc1083 bc1084 bc1085 bc1086 bc1087 bc1088 bc1089
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## bc1090 bc1091 bc1092 bc1093 bc1094 bc1095 bc1096 bc1097 bc1098 bc1099 bc1100
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc1101 bc1102 bc1103 bc1104 bc1105 bc1106 bc1107 bc1108 bc1109 bc1110 bc1111
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## bc1112 bc1113 bc1114 bc1115 bc1116 bc1117 bc1118 bc1119 bc1120 bc1121 bc1122
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## bc1200 bc1201 bc1202 bc1203 bc1204 bc1205 bc1206 bc1207 bc1208 bc1209 bc1210
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## bc1904 bc1905 bc1906 bc1907 bc1908 bc1909 bc1910 bc1911 bc1912 bc1913 bc1914
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## bc1926 bc1927 bc1928 bc1929 bc1930 bc1931 bc1932 bc1933 bc1934 bc1935 bc1936
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc1937 bc1938 bc1939 bc1940 bc1941 bc1942 bc1943 bc1944 bc1945 bc1946 bc1947
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## bc1948 bc1949 bc1950 bc1951 bc1952 bc1953 bc1954 bc1955 bc1956 bc1957 bc1958
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## bc1959 bc1960 bc1961 bc1962 bc1963 bc1964 bc1965 bc1966 bc1967 bc1968 bc1969
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## bc1970 bc1971 bc1972 bc1973 bc1974 bc1975 bc1976 bc1977 bc1978 bc1979 bc1980
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## bc1981 bc1982 bc1983 bc1984 bc1985 bc1986 bc1987 bc1988 bc1989 bc1990 bc1991
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## bc1992 bc1993 bc1994 bc1995 bc1996 bc1997 bc1998 bc1999 bc2000 bc2001 bc2002
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## bc2003 bc2004 bc2005 bc2006 bc2007 bc2008 bc2009 bc2010 bc2011 bc2012 bc2013
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## bc2014 bc2015 bc2016 bc2017 bc2018 bc2019 bc2020 bc2021 bc2022 bc2023 bc2024
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## bc2025 bc2026 bc2027 bc2028 bc2029 bc2030 bc2031 bc2032 bc2033 bc2034 bc2035
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## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3543 bc3544 bc3545 bc3546 bc3547 bc3548 bc3549 bc3550 bc3551 bc3552 bc3553
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3554 bc3555 bc3556 bc3557 bc3558 bc3559 bc3560 bc3561 bc3562 bc3563 bc3564
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3565 bc3566 bc3567 bc3568 bc3569 bc3570 bc3571 bc3572 bc3573 bc3574 bc3575
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3576 bc3577 bc3578 bc3579 bc3580 bc3581 bc3582 bc3583 bc3584 bc3585 bc3586
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3587 bc3588 bc3589 bc3590 bc3591 bc3592 bc3593 bc3594 bc3595 bc3596 bc3597
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3598 bc3599 bc3600 bc3601 bc3602 bc3603 bc3604 bc3605 bc3606 bc3607 bc3608
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3609 bc3610 bc3611 bc3612 bc3613 bc3614 bc3615 bc3616 bc3617 bc3618 bc3619
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3620 bc3621 bc3622 bc3623 bc3624 bc3625 bc3626 bc3627 bc3628 bc3629 bc3630
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3631 bc3632 bc3633 bc3634 bc3635 bc3636 bc3637 bc3638 bc3639 bc3640 bc3641
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3642 bc3643 bc3644 bc3645 bc3646 bc3647 bc3648 bc3649 bc3650 bc3651 bc3652
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3653 bc3654 bc3655 bc3656 bc3657 bc3658 bc3659 bc3660 bc3661 bc3662 bc3663
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3664 bc3665 bc3666 bc3667 bc3668 bc3669 bc3670 bc3671 bc3672 bc3673 bc3674
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3675 bc3676 bc3677 bc3678 bc3679 bc3680 bc3681 bc3682 bc3683 bc3684 bc3685
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3686 bc3687 bc3688 bc3689 bc3690 bc3691 bc3692 bc3693 bc3694 bc3695 bc3696
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3697 bc3698 bc3699 bc3700 bc3701 bc3702 bc3703 bc3704 bc3705 bc3706 bc3707
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3708 bc3709 bc3710 bc3711 bc3712 bc3713 bc3714 bc3715 bc3716 bc3717 bc3718
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3719 bc3720 bc3721 bc3722 bc3723 bc3724 bc3725 bc3726 bc3727 bc3728 bc3729
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3730 bc3731 bc3732 bc3733 bc3734 bc3735 bc3736 bc3737 bc3738 bc3739 bc3740
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3741 bc3742 bc3743 bc3744 bc3745 bc3746 bc3747 bc3748 bc3749 bc3750 bc3751
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3752 bc3753 bc3754 bc3755 bc3756 bc3757 bc3758 bc3759 bc3760 bc3761 bc3762
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3763 bc3764 bc3765 bc3766 bc3767 bc3768 bc3769 bc3770 bc3771 bc3772 bc3773
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3774 bc3775 bc3776 bc3777 bc3778 bc3779 bc3780 bc3781 bc3782 bc3783 bc3784
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3785 bc3786 bc3787 bc3788 bc3789 bc3790 bc3791 bc3792 bc3793 bc3794 bc3795
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3796 bc3797 bc3798 bc3799 bc3800 bc3801 bc3802 bc3803 bc3804 bc3805 bc3806
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3807 bc3808 bc3809 bc3810 bc3811 bc3812 bc3813 bc3814 bc3815 bc3816 bc3817
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3818 bc3819 bc3820 bc3821 bc3822 bc3823 bc3824 bc3825 bc3826 bc3827 bc3828
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## bc3829 bc3830 bc3831 bc3832 bc3833 bc3834 bc3835 bc3836 bc3837 bc3838
## YuC YuC YuC YuC YuC YuC YuC YuC YuC YuC
## Levels: YuCYuC$orig.ident <- "YuC"
YuT <- data.table::fread("/home/moyra.lawrence/R_analysis/Comparison/Yu/GSE166027_single_cell_gene_expression_CX5461(1).csv.gz", data.table = FALSE)
names(YuT)[2] <- 'Gene'
YuT <- YuT[,-1]
YuT <- YuT[,-2]
YuT <- YuT[,-2]
YuT <- YuT[,-2]
gene4 <- YuT$Gene
gene5 <- str_replace(gene4,
pattern = ".[0-9]+$",
replacement = "")
ens3 = getBM(attributes=c('ensembl_gene_id', 'mgi_symbol'),
filters = 'ensembl_gene_id',
values = gene5,
mart = ensembl)
YuT$Gene <- gene5
merge3 <- merge(YuT, ens2, by.x="Gene", by.y = "ensembl_gene_id")
merge3["mgi_symbol"][merge3["mgi_symbol"] == ''] <- "Unknown"
names3 <- make.unique(merge3$mgi_symbol)
names3 <- gsub("_", "-", names3)
names3 <- gsub("\\.", "-", names3)
names3 <- make.unique(names3)
rownames(merge3) <- names3
merge3 <- merge3[,-1]
merge3 <- merge3[,-4923]
YuT <- CreateSeuratObject(counts=merge3, project="YuT")
YuT$orig.ident## bc0001 bc0002 bc0003 bc0004 bc0005 bc0006 bc0007 bc0008 bc0009 bc0010 bc0011
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0012 bc0013 bc0014 bc0015 bc0016 bc0017 bc0018 bc0019 bc0020 bc0021 bc0022
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0023 bc0024 bc0025 bc0026 bc0027 bc0028 bc0029 bc0030 bc0031 bc0032 bc0033
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0034 bc0035 bc0036 bc0037 bc0038 bc0039 bc0040 bc0041 bc0042 bc0043 bc0044
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0045 bc0046 bc0047 bc0048 bc0049 bc0050 bc0051 bc0052 bc0053 bc0054 bc0055
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0056 bc0057 bc0058 bc0059 bc0060 bc0061 bc0062 bc0063 bc0064 bc0065 bc0066
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0067 bc0068 bc0069 bc0070 bc0071 bc0072 bc0073 bc0074 bc0075 bc0076 bc0077
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0078 bc0079 bc0080 bc0081 bc0082 bc0083 bc0084 bc0085 bc0086 bc0087 bc0088
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0089 bc0090 bc0091 bc0092 bc0093 bc0094 bc0095 bc0096 bc0097 bc0098 bc0099
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0100 bc0101 bc0102 bc0103 bc0104 bc0105 bc0106 bc0107 bc0108 bc0109 bc0110
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0111 bc0112 bc0113 bc0114 bc0115 bc0116 bc0117 bc0118 bc0119 bc0120 bc0121
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0122 bc0123 bc0124 bc0125 bc0126 bc0127 bc0128 bc0129 bc0130 bc0131 bc0132
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0133 bc0134 bc0135 bc0136 bc0137 bc0138 bc0139 bc0140 bc0141 bc0142 bc0143
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0144 bc0145 bc0146 bc0147 bc0148 bc0149 bc0150 bc0151 bc0152 bc0153 bc0154
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0155 bc0156 bc0157 bc0158 bc0159 bc0160 bc0161 bc0162 bc0163 bc0164 bc0165
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0166 bc0167 bc0168 bc0169 bc0170 bc0171 bc0172 bc0173 bc0174 bc0175 bc0176
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0177 bc0178 bc0179 bc0180 bc0181 bc0182 bc0183 bc0184 bc0185 bc0186 bc0187
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0188 bc0189 bc0190 bc0191 bc0192 bc0193 bc0194 bc0195 bc0196 bc0197 bc0198
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0199 bc0200 bc0201 bc0202 bc0203 bc0204 bc0205 bc0206 bc0207 bc0208 bc0209
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0210 bc0211 bc0212 bc0213 bc0214 bc0215 bc0216 bc0217 bc0218 bc0219 bc0220
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0221 bc0222 bc0223 bc0224 bc0225 bc0226 bc0227 bc0228 bc0229 bc0230 bc0231
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0232 bc0233 bc0234 bc0235 bc0236 bc0237 bc0238 bc0239 bc0240 bc0241 bc0242
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0243 bc0244 bc0245 bc0246 bc0247 bc0248 bc0249 bc0250 bc0251 bc0252 bc0253
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0254 bc0255 bc0256 bc0257 bc0258 bc0259 bc0260 bc0261 bc0262 bc0263 bc0264
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0265 bc0266 bc0267 bc0268 bc0269 bc0270 bc0271 bc0272 bc0273 bc0274 bc0275
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0276 bc0277 bc0278 bc0279 bc0280 bc0281 bc0282 bc0283 bc0284 bc0285 bc0286
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0287 bc0288 bc0289 bc0290 bc0291 bc0292 bc0293 bc0294 bc0295 bc0296 bc0297
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0298 bc0299 bc0300 bc0301 bc0302 bc0303 bc0304 bc0305 bc0306 bc0307 bc0308
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0309 bc0310 bc0311 bc0312 bc0313 bc0314 bc0315 bc0316 bc0317 bc0318 bc0319
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0320 bc0321 bc0322 bc0323 bc0324 bc0325 bc0326 bc0327 bc0328 bc0329 bc0330
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0331 bc0332 bc0333 bc0334 bc0335 bc0336 bc0337 bc0338 bc0339 bc0340 bc0341
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0342 bc0343 bc0344 bc0345 bc0346 bc0347 bc0348 bc0349 bc0350 bc0351 bc0352
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0353 bc0354 bc0355 bc0356 bc0357 bc0358 bc0359 bc0360 bc0361 bc0362 bc0363
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0364 bc0365 bc0366 bc0367 bc0368 bc0369 bc0370 bc0371 bc0372 bc0373 bc0374
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0375 bc0376 bc0377 bc0378 bc0379 bc0380 bc0381 bc0382 bc0383 bc0384 bc0385
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0386 bc0387 bc0388 bc0389 bc0390 bc0391 bc0392 bc0393 bc0394 bc0395 bc0396
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0397 bc0398 bc0399 bc0400 bc0401 bc0402 bc0403 bc0404 bc0405 bc0406 bc0407
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0408 bc0409 bc0410 bc0411 bc0412 bc0413 bc0414 bc0415 bc0416 bc0417 bc0418
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0419 bc0420 bc0421 bc0422 bc0423 bc0424 bc0425 bc0426 bc0427 bc0428 bc0429
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0430 bc0431 bc0432 bc0433 bc0434 bc0435 bc0436 bc0437 bc0438 bc0439 bc0440
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0441 bc0442 bc0443 bc0444 bc0445 bc0446 bc0447 bc0448 bc0449 bc0450 bc0451
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0452 bc0453 bc0454 bc0455 bc0456 bc0457 bc0458 bc0459 bc0460 bc0461 bc0462
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0463 bc0464 bc0465 bc0466 bc0467 bc0468 bc0469 bc0470 bc0471 bc0472 bc0473
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0474 bc0475 bc0476 bc0477 bc0478 bc0479 bc0480 bc0481 bc0482 bc0483 bc0484
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0485 bc0486 bc0487 bc0488 bc0489 bc0490 bc0491 bc0492 bc0493 bc0494 bc0495
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0496 bc0497 bc0498 bc0499 bc0500 bc0501 bc0502 bc0503 bc0504 bc0505 bc0506
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0507 bc0508 bc0509 bc0510 bc0511 bc0512 bc0513 bc0514 bc0515 bc0516 bc0517
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0518 bc0519 bc0520 bc0521 bc0522 bc0523 bc0524 bc0525 bc0526 bc0527 bc0528
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0529 bc0530 bc0531 bc0532 bc0533 bc0534 bc0535 bc0536 bc0537 bc0538 bc0539
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0540 bc0541 bc0542 bc0543 bc0544 bc0545 bc0546 bc0547 bc0548 bc0549 bc0550
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0551 bc0552 bc0553 bc0554 bc0555 bc0556 bc0557 bc0558 bc0559 bc0560 bc0561
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0562 bc0563 bc0564 bc0565 bc0566 bc0567 bc0568 bc0569 bc0570 bc0571 bc0572
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0573 bc0574 bc0575 bc0576 bc0577 bc0578 bc0579 bc0580 bc0581 bc0582 bc0583
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0584 bc0585 bc0586 bc0587 bc0588 bc0589 bc0590 bc0591 bc0592 bc0593 bc0594
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0595 bc0596 bc0597 bc0598 bc0599 bc0600 bc0601 bc0602 bc0603 bc0604 bc0605
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0606 bc0607 bc0608 bc0609 bc0610 bc0611 bc0612 bc0613 bc0614 bc0615 bc0616
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0617 bc0618 bc0619 bc0620 bc0621 bc0622 bc0623 bc0624 bc0625 bc0626 bc0627
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0628 bc0629 bc0630 bc0631 bc0632 bc0633 bc0634 bc0635 bc0636 bc0637 bc0638
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0639 bc0640 bc0641 bc0642 bc0643 bc0644 bc0645 bc0646 bc0647 bc0648 bc0649
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0650 bc0651 bc0652 bc0653 bc0654 bc0655 bc0656 bc0657 bc0658 bc0659 bc0660
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0661 bc0662 bc0663 bc0664 bc0665 bc0666 bc0667 bc0668 bc0669 bc0670 bc0671
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0672 bc0673 bc0674 bc0675 bc0676 bc0677 bc0678 bc0679 bc0680 bc0681 bc0682
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0683 bc0684 bc0685 bc0686 bc0687 bc0688 bc0689 bc0690 bc0691 bc0692 bc0693
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0694 bc0695 bc0696 bc0697 bc0698 bc0699 bc0700 bc0701 bc0702 bc0703 bc0704
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0705 bc0706 bc0707 bc0708 bc0709 bc0710 bc0711 bc0712 bc0713 bc0714 bc0715
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0716 bc0717 bc0718 bc0719 bc0720 bc0721 bc0722 bc0723 bc0724 bc0725 bc0726
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0727 bc0728 bc0729 bc0730 bc0731 bc0732 bc0733 bc0734 bc0735 bc0736 bc0737
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0738 bc0739 bc0740 bc0741 bc0742 bc0743 bc0744 bc0745 bc0746 bc0747 bc0748
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0749 bc0750 bc0751 bc0752 bc0753 bc0754 bc0755 bc0756 bc0757 bc0758 bc0759
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0760 bc0761 bc0762 bc0763 bc0764 bc0765 bc0766 bc0767 bc0768 bc0769 bc0770
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0771 bc0772 bc0773 bc0774 bc0775 bc0776 bc0777 bc0778 bc0779 bc0780 bc0781
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0782 bc0783 bc0784 bc0785 bc0786 bc0787 bc0788 bc0789 bc0790 bc0791 bc0792
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0793 bc0794 bc0795 bc0796 bc0797 bc0798 bc0799 bc0800 bc0801 bc0802 bc0803
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0804 bc0805 bc0806 bc0807 bc0808 bc0809 bc0810 bc0811 bc0812 bc0813 bc0814
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0815 bc0816 bc0817 bc0818 bc0819 bc0820 bc0821 bc0822 bc0823 bc0824 bc0825
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0826 bc0827 bc0828 bc0829 bc0830 bc0831 bc0832 bc0833 bc0834 bc0835 bc0836
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0837 bc0838 bc0839 bc0840 bc0841 bc0842 bc0843 bc0844 bc0845 bc0846 bc0847
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0848 bc0849 bc0850 bc0851 bc0852 bc0853 bc0854 bc0855 bc0856 bc0857 bc0858
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0859 bc0860 bc0861 bc0862 bc0863 bc0864 bc0865 bc0866 bc0867 bc0868 bc0869
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0870 bc0871 bc0872 bc0873 bc0874 bc0875 bc0876 bc0877 bc0878 bc0879 bc0880
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0881 bc0882 bc0883 bc0884 bc0885 bc0886 bc0887 bc0888 bc0889 bc0890 bc0891
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0892 bc0893 bc0894 bc0895 bc0896 bc0897 bc0898 bc0899 bc0900 bc0901 bc0902
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0903 bc0904 bc0905 bc0906 bc0907 bc0908 bc0909 bc0910 bc0911 bc0912 bc0913
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0914 bc0915 bc0916 bc0917 bc0918 bc0919 bc0920 bc0921 bc0922 bc0923 bc0924
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0925 bc0926 bc0927 bc0928 bc0929 bc0930 bc0931 bc0932 bc0933 bc0934 bc0935
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0936 bc0937 bc0938 bc0939 bc0940 bc0941 bc0942 bc0943 bc0944 bc0945 bc0946
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0947 bc0948 bc0949 bc0950 bc0951 bc0952 bc0953 bc0954 bc0955 bc0956 bc0957
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0958 bc0959 bc0960 bc0961 bc0962 bc0963 bc0964 bc0965 bc0966 bc0967 bc0968
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0969 bc0970 bc0971 bc0972 bc0973 bc0974 bc0975 bc0976 bc0977 bc0978 bc0979
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0980 bc0981 bc0982 bc0983 bc0984 bc0985 bc0986 bc0987 bc0988 bc0989 bc0990
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc0991 bc0992 bc0993 bc0994 bc0995 bc0996 bc0997 bc0998 bc0999 bc1000 bc1001
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1002 bc1003 bc1004 bc1005 bc1006 bc1007 bc1008 bc1009 bc1010 bc1011 bc1012
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1013 bc1014 bc1015 bc1016 bc1017 bc1018 bc1019 bc1020 bc1021 bc1022 bc1023
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1024 bc1025 bc1026 bc1027 bc1028 bc1029 bc1030 bc1031 bc1032 bc1033 bc1034
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1035 bc1036 bc1037 bc1038 bc1039 bc1040 bc1041 bc1042 bc1043 bc1044 bc1045
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1046 bc1047 bc1048 bc1049 bc1050 bc1051 bc1052 bc1053 bc1054 bc1055 bc1056
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1057 bc1058 bc1059 bc1060 bc1061 bc1062 bc1063 bc1064 bc1065 bc1066 bc1067
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1068 bc1069 bc1070 bc1071 bc1072 bc1073 bc1074 bc1075 bc1076 bc1077 bc1078
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1079 bc1080 bc1081 bc1082 bc1083 bc1084 bc1085 bc1086 bc1087 bc1088 bc1089
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1090 bc1091 bc1092 bc1093 bc1094 bc1095 bc1096 bc1097 bc1098 bc1099 bc1100
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1101 bc1102 bc1103 bc1104 bc1105 bc1106 bc1107 bc1108 bc1109 bc1110 bc1111
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1112 bc1113 bc1114 bc1115 bc1116 bc1117 bc1118 bc1119 bc1120 bc1121 bc1122
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1123 bc1124 bc1125 bc1126 bc1127 bc1128 bc1129 bc1130 bc1131 bc1132 bc1133
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1134 bc1135 bc1136 bc1137 bc1138 bc1139 bc1140 bc1141 bc1142 bc1143 bc1144
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1145 bc1146 bc1147 bc1148 bc1149 bc1150 bc1151 bc1152 bc1153 bc1154 bc1155
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1156 bc1157 bc1158 bc1159 bc1160 bc1161 bc1162 bc1163 bc1164 bc1165 bc1166
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1167 bc1168 bc1169 bc1170 bc1171 bc1172 bc1173 bc1174 bc1175 bc1176 bc1177
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1178 bc1179 bc1180 bc1181 bc1182 bc1183 bc1184 bc1185 bc1186 bc1187 bc1188
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1189 bc1190 bc1191 bc1192 bc1193 bc1194 bc1195 bc1196 bc1197 bc1198 bc1199
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1200 bc1201 bc1202 bc1203 bc1204 bc1205 bc1206 bc1207 bc1208 bc1209 bc1210
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1211 bc1212 bc1213 bc1214 bc1215 bc1216 bc1217 bc1218 bc1219 bc1220 bc1221
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1222 bc1223 bc1224 bc1225 bc1226 bc1227 bc1228 bc1229 bc1230 bc1231 bc1232
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1233 bc1234 bc1235 bc1236 bc1237 bc1238 bc1239 bc1240 bc1241 bc1242 bc1243
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1244 bc1245 bc1246 bc1247 bc1248 bc1249 bc1250 bc1251 bc1252 bc1253 bc1254
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1255 bc1256 bc1257 bc1258 bc1259 bc1260 bc1261 bc1262 bc1263 bc1264 bc1265
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1266 bc1267 bc1268 bc1269 bc1270 bc1271 bc1272 bc1273 bc1274 bc1275 bc1276
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1277 bc1278 bc1279 bc1280 bc1281 bc1282 bc1283 bc1284 bc1285 bc1286 bc1287
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1288 bc1289 bc1290 bc1291 bc1292 bc1293 bc1294 bc1295 bc1296 bc1297 bc1298
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1299 bc1300 bc1301 bc1302 bc1303 bc1304 bc1305 bc1306 bc1307 bc1308 bc1309
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1310 bc1311 bc1312 bc1313 bc1314 bc1315 bc1316 bc1317 bc1318 bc1319 bc1320
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1321 bc1322 bc1323 bc1324 bc1325 bc1326 bc1327 bc1328 bc1329 bc1330 bc1331
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1332 bc1333 bc1334 bc1335 bc1336 bc1337 bc1338 bc1339 bc1340 bc1341 bc1342
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1343 bc1344 bc1345 bc1346 bc1347 bc1348 bc1349 bc1350 bc1351 bc1352 bc1353
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1354 bc1355 bc1356 bc1357 bc1358 bc1359 bc1360 bc1361 bc1362 bc1363 bc1364
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1365 bc1366 bc1367 bc1368 bc1369 bc1370 bc1371 bc1372 bc1373 bc1374 bc1375
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1376 bc1377 bc1378 bc1379 bc1380 bc1381 bc1382 bc1383 bc1384 bc1385 bc1386
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1387 bc1388 bc1389 bc1390 bc1391 bc1392 bc1393 bc1394 bc1395 bc1396 bc1397
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1398 bc1399 bc1400 bc1401 bc1402 bc1403 bc1404 bc1405 bc1406 bc1407 bc1408
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1409 bc1410 bc1411 bc1412 bc1413 bc1414 bc1415 bc1416 bc1417 bc1418 bc1419
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1420 bc1421 bc1422 bc1423 bc1424 bc1425 bc1426 bc1427 bc1428 bc1429 bc1430
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1431 bc1432 bc1433 bc1434 bc1435 bc1436 bc1437 bc1438 bc1439 bc1440 bc1441
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1442 bc1443 bc1444 bc1445 bc1446 bc1447 bc1448 bc1449 bc1450 bc1451 bc1452
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1453 bc1454 bc1455 bc1456 bc1457 bc1458 bc1459 bc1460 bc1461 bc1462 bc1463
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1464 bc1465 bc1466 bc1467 bc1468 bc1469 bc1470 bc1471 bc1472 bc1473 bc1474
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1475 bc1476 bc1477 bc1478 bc1479 bc1480 bc1481 bc1482 bc1483 bc1484 bc1485
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1486 bc1487 bc1488 bc1489 bc1490 bc1491 bc1492 bc1493 bc1494 bc1495 bc1496
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1497 bc1498 bc1499 bc1500 bc1501 bc1502 bc1503 bc1504 bc1505 bc1506 bc1507
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1508 bc1509 bc1510 bc1511 bc1512 bc1513 bc1514 bc1515 bc1516 bc1517 bc1518
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1519 bc1520 bc1521 bc1522 bc1523 bc1524 bc1525 bc1526 bc1527 bc1528 bc1529
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1530 bc1531 bc1532 bc1533 bc1534 bc1535 bc1536 bc1537 bc1538 bc1539 bc1540
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1541 bc1542 bc1543 bc1544 bc1545 bc1546 bc1547 bc1548 bc1549 bc1550 bc1551
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1552 bc1553 bc1554 bc1555 bc1556 bc1557 bc1558 bc1559 bc1560 bc1561 bc1562
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1563 bc1564 bc1565 bc1566 bc1567 bc1568 bc1569 bc1570 bc1571 bc1572 bc1573
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1574 bc1575 bc1576 bc1577 bc1578 bc1579 bc1580 bc1581 bc1582 bc1583 bc1584
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1585 bc1586 bc1587 bc1588 bc1589 bc1590 bc1591 bc1592 bc1593 bc1594 bc1595
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1596 bc1597 bc1598 bc1599 bc1600 bc1601 bc1602 bc1603 bc1604 bc1605 bc1606
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1607 bc1608 bc1609 bc1610 bc1611 bc1612 bc1613 bc1614 bc1615 bc1616 bc1617
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1618 bc1619 bc1620 bc1621 bc1622 bc1623 bc1624 bc1625 bc1626 bc1627 bc1628
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1629 bc1630 bc1631 bc1632 bc1633 bc1634 bc1635 bc1636 bc1637 bc1638 bc1639
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1640 bc1641 bc1642 bc1643 bc1644 bc1645 bc1646 bc1647 bc1648 bc1649 bc1650
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1651 bc1652 bc1653 bc1654 bc1655 bc1656 bc1657 bc1658 bc1659 bc1660 bc1661
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1662 bc1663 bc1664 bc1665 bc1666 bc1667 bc1668 bc1669 bc1670 bc1671 bc1672
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1673 bc1674 bc1675 bc1676 bc1677 bc1678 bc1679 bc1680 bc1681 bc1682 bc1683
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1684 bc1685 bc1686 bc1687 bc1688 bc1689 bc1690 bc1691 bc1692 bc1693 bc1694
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1695 bc1696 bc1697 bc1698 bc1699 bc1700 bc1701 bc1702 bc1703 bc1704 bc1705
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1706 bc1707 bc1708 bc1709 bc1710 bc1711 bc1712 bc1713 bc1714 bc1715 bc1716
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1717 bc1718 bc1719 bc1720 bc1721 bc1722 bc1723 bc1724 bc1725 bc1726 bc1727
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1728 bc1729 bc1730 bc1731 bc1732 bc1733 bc1734 bc1735 bc1736 bc1737 bc1738
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1739 bc1740 bc1741 bc1742 bc1743 bc1744 bc1745 bc1746 bc1747 bc1748 bc1749
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1750 bc1751 bc1752 bc1753 bc1754 bc1755 bc1756 bc1757 bc1758 bc1759 bc1760
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1761 bc1762 bc1763 bc1764 bc1765 bc1766 bc1767 bc1768 bc1769 bc1770 bc1771
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1772 bc1773 bc1774 bc1775 bc1776 bc1777 bc1778 bc1779 bc1780 bc1781 bc1782
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1783 bc1784 bc1785 bc1786 bc1787 bc1788 bc1789 bc1790 bc1791 bc1792 bc1793
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1794 bc1795 bc1796 bc1797 bc1798 bc1799 bc1800 bc1801 bc1802 bc1803 bc1804
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1805 bc1806 bc1807 bc1808 bc1809 bc1810 bc1811 bc1812 bc1813 bc1814 bc1815
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1816 bc1817 bc1818 bc1819 bc1820 bc1821 bc1822 bc1823 bc1824 bc1825 bc1826
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1827 bc1828 bc1829 bc1830 bc1831 bc1832 bc1833 bc1834 bc1835 bc1836 bc1837
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1838 bc1839 bc1840 bc1841 bc1842 bc1843 bc1844 bc1845 bc1846 bc1847 bc1848
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1849 bc1850 bc1851 bc1852 bc1853 bc1854 bc1855 bc1856 bc1857 bc1858 bc1859
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1860 bc1861 bc1862 bc1863 bc1864 bc1865 bc1866 bc1867 bc1868 bc1869 bc1870
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1871 bc1872 bc1873 bc1874 bc1875 bc1876 bc1877 bc1878 bc1879 bc1880 bc1881
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1882 bc1883 bc1884 bc1885 bc1886 bc1887 bc1888 bc1889 bc1890 bc1891 bc1892
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1893 bc1894 bc1895 bc1896 bc1897 bc1898 bc1899 bc1900 bc1901 bc1902 bc1903
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1904 bc1905 bc1906 bc1907 bc1908 bc1909 bc1910 bc1911 bc1912 bc1913 bc1914
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1915 bc1916 bc1917 bc1918 bc1919 bc1920 bc1921 bc1922 bc1923 bc1924 bc1925
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1926 bc1927 bc1928 bc1929 bc1930 bc1931 bc1932 bc1933 bc1934 bc1935 bc1936
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1937 bc1938 bc1939 bc1940 bc1941 bc1942 bc1943 bc1944 bc1945 bc1946 bc1947
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1948 bc1949 bc1950 bc1951 bc1952 bc1953 bc1954 bc1955 bc1956 bc1957 bc1958
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1959 bc1960 bc1961 bc1962 bc1963 bc1964 bc1965 bc1966 bc1967 bc1968 bc1969
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1970 bc1971 bc1972 bc1973 bc1974 bc1975 bc1976 bc1977 bc1978 bc1979 bc1980
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1981 bc1982 bc1983 bc1984 bc1985 bc1986 bc1987 bc1988 bc1989 bc1990 bc1991
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc1992 bc1993 bc1994 bc1995 bc1996 bc1997 bc1998 bc1999 bc2000 bc2001 bc2002
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2003 bc2004 bc2005 bc2006 bc2007 bc2008 bc2009 bc2010 bc2011 bc2012 bc2013
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2014 bc2015 bc2016 bc2017 bc2018 bc2019 bc2020 bc2021 bc2022 bc2023 bc2024
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2025 bc2026 bc2027 bc2028 bc2029 bc2030 bc2031 bc2032 bc2033 bc2034 bc2035
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2036 bc2037 bc2038 bc2039 bc2040 bc2041 bc2042 bc2043 bc2044 bc2045 bc2046
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2047 bc2048 bc2049 bc2050 bc2051 bc2052 bc2053 bc2054 bc2055 bc2056 bc2057
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2058 bc2059 bc2060 bc2061 bc2062 bc2063 bc2064 bc2065 bc2066 bc2067 bc2068
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2069 bc2070 bc2071 bc2072 bc2073 bc2074 bc2075 bc2076 bc2077 bc2078 bc2079
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2080 bc2081 bc2082 bc2083 bc2084 bc2085 bc2086 bc2087 bc2088 bc2089 bc2090
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2091 bc2092 bc2093 bc2094 bc2095 bc2096 bc2097 bc2098 bc2099 bc2100 bc2101
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2102 bc2103 bc2104 bc2105 bc2106 bc2107 bc2108 bc2109 bc2110 bc2111 bc2112
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2113 bc2114 bc2115 bc2116 bc2117 bc2118 bc2119 bc2120 bc2121 bc2122 bc2123
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2124 bc2125 bc2126 bc2127 bc2128 bc2129 bc2130 bc2131 bc2132 bc2133 bc2134
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2135 bc2136 bc2137 bc2138 bc2139 bc2140 bc2141 bc2142 bc2143 bc2144 bc2145
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2146 bc2147 bc2148 bc2149 bc2150 bc2151 bc2152 bc2153 bc2154 bc2155 bc2156
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2157 bc2158 bc2159 bc2160 bc2161 bc2162 bc2163 bc2164 bc2165 bc2166 bc2167
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2168 bc2169 bc2170 bc2171 bc2172 bc2173 bc2174 bc2175 bc2176 bc2177 bc2178
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2179 bc2180 bc2181 bc2182 bc2183 bc2184 bc2185 bc2186 bc2187 bc2188 bc2189
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2190 bc2191 bc2192 bc2193 bc2194 bc2195 bc2196 bc2197 bc2198 bc2199 bc2200
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2201 bc2202 bc2203 bc2204 bc2205 bc2206 bc2207 bc2208 bc2209 bc2210 bc2211
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2212 bc2213 bc2214 bc2215 bc2216 bc2217 bc2218 bc2219 bc2220 bc2221 bc2222
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2223 bc2224 bc2225 bc2226 bc2227 bc2228 bc2229 bc2230 bc2231 bc2232 bc2233
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2234 bc2235 bc2236 bc2237 bc2238 bc2239 bc2240 bc2241 bc2242 bc2243 bc2244
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2245 bc2246 bc2247 bc2248 bc2249 bc2250 bc2251 bc2252 bc2253 bc2254 bc2255
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2256 bc2257 bc2258 bc2259 bc2260 bc2261 bc2262 bc2263 bc2264 bc2265 bc2266
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2267 bc2268 bc2269 bc2270 bc2271 bc2272 bc2273 bc2274 bc2275 bc2276 bc2277
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2278 bc2279 bc2280 bc2281 bc2282 bc2283 bc2284 bc2285 bc2286 bc2287 bc2288
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2289 bc2290 bc2291 bc2292 bc2293 bc2294 bc2295 bc2296 bc2297 bc2298 bc2299
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2300 bc2301 bc2302 bc2303 bc2304 bc2305 bc2306 bc2307 bc2308 bc2309 bc2310
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2311 bc2312 bc2313 bc2314 bc2315 bc2316 bc2317 bc2318 bc2319 bc2320 bc2321
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2322 bc2323 bc2324 bc2325 bc2326 bc2327 bc2328 bc2329 bc2330 bc2331 bc2332
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2333 bc2334 bc2335 bc2336 bc2337 bc2338 bc2339 bc2340 bc2341 bc2342 bc2343
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2344 bc2345 bc2346 bc2347 bc2348 bc2349 bc2350 bc2351 bc2352 bc2353 bc2354
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2355 bc2356 bc2357 bc2358 bc2359 bc2360 bc2361 bc2362 bc2363 bc2364 bc2365
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2366 bc2367 bc2368 bc2369 bc2370 bc2371 bc2372 bc2373 bc2374 bc2375 bc2376
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2377 bc2378 bc2379 bc2380 bc2381 bc2382 bc2383 bc2384 bc2385 bc2386 bc2387
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2388 bc2389 bc2390 bc2391 bc2392 bc2393 bc2394 bc2395 bc2396 bc2397 bc2398
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2399 bc2400 bc2401 bc2402 bc2403 bc2404 bc2405 bc2406 bc2407 bc2408 bc2409
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2410 bc2411 bc2412 bc2413 bc2414 bc2415 bc2416 bc2417 bc2418 bc2419 bc2420
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2421 bc2422 bc2423 bc2424 bc2425 bc2426 bc2427 bc2428 bc2429 bc2430 bc2431
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2432 bc2433 bc2434 bc2435 bc2436 bc2437 bc2438 bc2439 bc2440 bc2441 bc2442
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2443 bc2444 bc2445 bc2446 bc2447 bc2448 bc2449 bc2450 bc2451 bc2452 bc2453
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2454 bc2455 bc2456 bc2457 bc2458 bc2459 bc2460 bc2461 bc2462 bc2463 bc2464
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2465 bc2466 bc2467 bc2468 bc2469 bc2470 bc2471 bc2472 bc2473 bc2474 bc2475
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2476 bc2477 bc2478 bc2479 bc2480 bc2481 bc2482 bc2483 bc2484 bc2485 bc2486
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2487 bc2488 bc2489 bc2490 bc2491 bc2492 bc2493 bc2494 bc2495 bc2496 bc2497
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2498 bc2499 bc2500 bc2501 bc2502 bc2503 bc2504 bc2505 bc2506 bc2507 bc2508
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2509 bc2510 bc2511 bc2512 bc2513 bc2514 bc2515 bc2516 bc2517 bc2518 bc2519
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2520 bc2521 bc2522 bc2523 bc2524 bc2525 bc2526 bc2527 bc2528 bc2529 bc2530
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2531 bc2532 bc2533 bc2534 bc2535 bc2536 bc2537 bc2538 bc2539 bc2540 bc2541
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2542 bc2543 bc2544 bc2545 bc2546 bc2547 bc2548 bc2549 bc2550 bc2551 bc2552
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2553 bc2554 bc2555 bc2556 bc2557 bc2558 bc2559 bc2560 bc2561 bc2562 bc2563
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2564 bc2565 bc2566 bc2567 bc2568 bc2569 bc2570 bc2571 bc2572 bc2573 bc2574
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2575 bc2576 bc2577 bc2578 bc2579 bc2580 bc2581 bc2582 bc2583 bc2584 bc2585
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2586 bc2587 bc2588 bc2589 bc2590 bc2591 bc2592 bc2593 bc2594 bc2595 bc2596
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2597 bc2598 bc2599 bc2600 bc2601 bc2602 bc2603 bc2604 bc2605 bc2606 bc2607
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2608 bc2609 bc2610 bc2611 bc2612 bc2613 bc2614 bc2615 bc2616 bc2617 bc2618
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2619 bc2620 bc2621 bc2622 bc2623 bc2624 bc2625 bc2626 bc2627 bc2628 bc2629
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2630 bc2631 bc2632 bc2633 bc2634 bc2635 bc2636 bc2637 bc2638 bc2639 bc2640
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2641 bc2642 bc2643 bc2644 bc2645 bc2646 bc2647 bc2648 bc2649 bc2650 bc2651
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2652 bc2653 bc2654 bc2655 bc2656 bc2657 bc2658 bc2659 bc2660 bc2661 bc2662
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2663 bc2664 bc2665 bc2666 bc2667 bc2668 bc2669 bc2670 bc2671 bc2672 bc2673
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2674 bc2675 bc2676 bc2677 bc2678 bc2679 bc2680 bc2681 bc2682 bc2683 bc2684
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2685 bc2686 bc2687 bc2688 bc2689 bc2690 bc2691 bc2692 bc2693 bc2694 bc2695
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2696 bc2697 bc2698 bc2699 bc2700 bc2701 bc2702 bc2703 bc2704 bc2705 bc2706
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2707 bc2708 bc2709 bc2710 bc2711 bc2712 bc2713 bc2714 bc2715 bc2716 bc2717
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2718 bc2719 bc2720 bc2721 bc2722 bc2723 bc2724 bc2725 bc2726 bc2727 bc2728
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2729 bc2730 bc2731 bc2732 bc2733 bc2734 bc2735 bc2736 bc2737 bc2738 bc2739
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2740 bc2741 bc2742 bc2743 bc2744 bc2745 bc2746 bc2747 bc2748 bc2749 bc2750
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2751 bc2752 bc2753 bc2754 bc2755 bc2756 bc2757 bc2758 bc2759 bc2760 bc2761
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2762 bc2763 bc2764 bc2765 bc2766 bc2767 bc2768 bc2769 bc2770 bc2771 bc2772
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2773 bc2774 bc2775 bc2776 bc2777 bc2778 bc2779 bc2780 bc2781 bc2782 bc2783
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2784 bc2785 bc2786 bc2787 bc2788 bc2789 bc2790 bc2791 bc2792 bc2793 bc2794
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2795 bc2796 bc2797 bc2798 bc2799 bc2800 bc2801 bc2802 bc2803 bc2804 bc2805
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2806 bc2807 bc2808 bc2809 bc2810 bc2811 bc2812 bc2813 bc2814 bc2815 bc2816
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2817 bc2818 bc2819 bc2820 bc2821 bc2822 bc2823 bc2824 bc2825 bc2826 bc2827
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2828 bc2829 bc2830 bc2831 bc2832 bc2833 bc2834 bc2835 bc2836 bc2837 bc2838
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2839 bc2840 bc2841 bc2842 bc2843 bc2844 bc2845 bc2846 bc2847 bc2848 bc2849
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2850 bc2851 bc2852 bc2853 bc2854 bc2855 bc2856 bc2857 bc2858 bc2859 bc2860
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2861 bc2862 bc2863 bc2864 bc2865 bc2866 bc2867 bc2868 bc2869 bc2870 bc2871
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2872 bc2873 bc2874 bc2875 bc2876 bc2877 bc2878 bc2879 bc2880 bc2881 bc2882
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2883 bc2884 bc2885 bc2886 bc2887 bc2888 bc2889 bc2890 bc2891 bc2892 bc2893
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2894 bc2895 bc2896 bc2897 bc2898 bc2899 bc2900 bc2901 bc2902 bc2903 bc2904
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2905 bc2906 bc2907 bc2908 bc2909 bc2910 bc2911 bc2912 bc2913 bc2914 bc2915
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2916 bc2917 bc2918 bc2919 bc2920 bc2921 bc2922 bc2923 bc2924 bc2925 bc2926
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2927 bc2928 bc2929 bc2930 bc2931 bc2932 bc2933 bc2934 bc2935 bc2936 bc2937
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2938 bc2939 bc2940 bc2941 bc2942 bc2943 bc2944 bc2945 bc2946 bc2947 bc2948
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2949 bc2950 bc2951 bc2952 bc2953 bc2954 bc2955 bc2956 bc2957 bc2958 bc2959
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2960 bc2961 bc2962 bc2963 bc2964 bc2965 bc2966 bc2967 bc2968 bc2969 bc2970
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2971 bc2972 bc2973 bc2974 bc2975 bc2976 bc2977 bc2978 bc2979 bc2980 bc2981
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2982 bc2983 bc2984 bc2985 bc2986 bc2987 bc2988 bc2989 bc2990 bc2991 bc2992
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc2993 bc2994 bc2995 bc2996 bc2997 bc2998 bc2999 bc3000 bc3001 bc3002 bc3003
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3004 bc3005 bc3006 bc3007 bc3008 bc3009 bc3010 bc3011 bc3012 bc3013 bc3014
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3015 bc3016 bc3017 bc3018 bc3019 bc3020 bc3021 bc3022 bc3023 bc3024 bc3025
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3026 bc3027 bc3028 bc3029 bc3030 bc3031 bc3032 bc3033 bc3034 bc3035 bc3036
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3037 bc3038 bc3039 bc3040 bc3041 bc3042 bc3043 bc3044 bc3045 bc3046 bc3047
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3048 bc3049 bc3050 bc3051 bc3052 bc3053 bc3054 bc3055 bc3056 bc3057 bc3058
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3059 bc3060 bc3061 bc3062 bc3063 bc3064 bc3065 bc3066 bc3067 bc3068 bc3069
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3070 bc3071 bc3072 bc3073 bc3074 bc3075 bc3076 bc3077 bc3078 bc3079 bc3080
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3081 bc3082 bc3083 bc3084 bc3085 bc3086 bc3087 bc3088 bc3089 bc3090 bc3091
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3092 bc3093 bc3094 bc3095 bc3096 bc3097 bc3098 bc3099 bc3100 bc3101 bc3102
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3103 bc3104 bc3105 bc3106 bc3107 bc3108 bc3109 bc3110 bc3111 bc3112 bc3113
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3114 bc3115 bc3116 bc3117 bc3118 bc3119 bc3120 bc3121 bc3122 bc3123 bc3124
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3125 bc3126 bc3127 bc3128 bc3129 bc3130 bc3131 bc3132 bc3133 bc3134 bc3135
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3136 bc3137 bc3138 bc3139 bc3140 bc3141 bc3142 bc3143 bc3144 bc3145 bc3146
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3147 bc3148 bc3149 bc3150 bc3151 bc3152 bc3153 bc3154 bc3155 bc3156 bc3157
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3158 bc3159 bc3160 bc3161 bc3162 bc3163 bc3164 bc3165 bc3166 bc3167 bc3168
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3169 bc3170 bc3171 bc3172 bc3173 bc3174 bc3175 bc3176 bc3177 bc3178 bc3179
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3180 bc3181 bc3182 bc3183 bc3184 bc3185 bc3186 bc3187 bc3188 bc3189 bc3190
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3191 bc3192 bc3193 bc3194 bc3195 bc3196 bc3197 bc3198 bc3199 bc3200 bc3201
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3202 bc3203 bc3204 bc3205 bc3206 bc3207 bc3208 bc3209 bc3210 bc3211 bc3212
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3213 bc3214 bc3215 bc3216 bc3217 bc3218 bc3219 bc3220 bc3221 bc3222 bc3223
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3224 bc3225 bc3226 bc3227 bc3228 bc3229 bc3230 bc3231 bc3232 bc3233 bc3234
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3235 bc3236 bc3237 bc3238 bc3239 bc3240 bc3241 bc3242 bc3243 bc3244 bc3245
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3246 bc3247 bc3248 bc3249 bc3250 bc3251 bc3252 bc3253 bc3254 bc3255 bc3256
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3257 bc3258 bc3259 bc3260 bc3261 bc3262 bc3263 bc3264 bc3265 bc3266 bc3267
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3268 bc3269 bc3270 bc3271 bc3272 bc3273 bc3274 bc3275 bc3276 bc3277 bc3278
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3279 bc3280 bc3281 bc3282 bc3283 bc3284 bc3285 bc3286 bc3287 bc3288 bc3289
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3290 bc3291 bc3292 bc3293 bc3294 bc3295 bc3296 bc3297 bc3298 bc3299 bc3300
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3301 bc3302 bc3303 bc3304 bc3305 bc3306 bc3307 bc3308 bc3309 bc3310 bc3311
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3312 bc3313 bc3314 bc3315 bc3316 bc3317 bc3318 bc3319 bc3320 bc3321 bc3322
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3323 bc3324 bc3325 bc3326 bc3327 bc3328 bc3329 bc3330 bc3331 bc3332 bc3333
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3334 bc3335 bc3336 bc3337 bc3338 bc3339 bc3340 bc3341 bc3342 bc3343 bc3344
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3345 bc3346 bc3347 bc3348 bc3349 bc3350 bc3351 bc3352 bc3353 bc3354 bc3355
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3356 bc3357 bc3358 bc3359 bc3360 bc3361 bc3362 bc3363 bc3364 bc3365 bc3366
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3367 bc3368 bc3369 bc3370 bc3371 bc3372 bc3373 bc3374 bc3375 bc3376 bc3377
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3378 bc3379 bc3380 bc3381 bc3382 bc3383 bc3384 bc3385 bc3386 bc3387 bc3388
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3389 bc3390 bc3391 bc3392 bc3393 bc3394 bc3395 bc3396 bc3397 bc3398 bc3399
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3400 bc3401 bc3402 bc3403 bc3404 bc3405 bc3406 bc3407 bc3408 bc3409 bc3410
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3411 bc3412 bc3413 bc3414 bc3415 bc3416 bc3417 bc3418 bc3419 bc3420 bc3421
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3422 bc3423 bc3424 bc3425 bc3426 bc3427 bc3428 bc3429 bc3430 bc3431 bc3432
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3433 bc3434 bc3435 bc3436 bc3437 bc3438 bc3439 bc3440 bc3441 bc3442 bc3443
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3444 bc3445 bc3446 bc3447 bc3448 bc3449 bc3450 bc3451 bc3452 bc3453 bc3454
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3455 bc3456 bc3457 bc3458 bc3459 bc3460 bc3461 bc3462 bc3463 bc3464 bc3465
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3466 bc3467 bc3468 bc3469 bc3470 bc3471 bc3472 bc3473 bc3474 bc3475 bc3476
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3477 bc3478 bc3479 bc3480 bc3481 bc3482 bc3483 bc3484 bc3485 bc3486 bc3487
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3488 bc3489 bc3490 bc3491 bc3492 bc3493 bc3494 bc3495 bc3496 bc3497 bc3498
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3499 bc3500 bc3501 bc3502 bc3503 bc3504 bc3505 bc3506 bc3507 bc3508 bc3509
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3510 bc3511 bc3512 bc3513 bc3514 bc3515 bc3516 bc3517 bc3518 bc3519 bc3520
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3521 bc3522 bc3523 bc3524 bc3525 bc3526 bc3527 bc3528 bc3529 bc3530 bc3531
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3532 bc3533 bc3534 bc3535 bc3536 bc3537 bc3538 bc3539 bc3540 bc3541 bc3542
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3543 bc3544 bc3545 bc3546 bc3547 bc3548 bc3549 bc3550 bc3551 bc3552 bc3553
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3554 bc3555 bc3556 bc3557 bc3558 bc3559 bc3560 bc3561 bc3562 bc3563 bc3564
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3565 bc3566 bc3567 bc3568 bc3569 bc3570 bc3571 bc3572 bc3573 bc3574 bc3575
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3576 bc3577 bc3578 bc3579 bc3580 bc3581 bc3582 bc3583 bc3584 bc3585 bc3586
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3587 bc3588 bc3589 bc3590 bc3591 bc3592 bc3593 bc3594 bc3595 bc3596 bc3597
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3598 bc3599 bc3600 bc3601 bc3602 bc3603 bc3604 bc3605 bc3606 bc3607 bc3608
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3609 bc3610 bc3611 bc3612 bc3613 bc3614 bc3615 bc3616 bc3617 bc3618 bc3619
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3620 bc3621 bc3622 bc3623 bc3624 bc3625 bc3626 bc3627 bc3628 bc3629 bc3630
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3631 bc3632 bc3633 bc3634 bc3635 bc3636 bc3637 bc3638 bc3639 bc3640 bc3641
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3642 bc3643 bc3644 bc3645 bc3646 bc3647 bc3648 bc3649 bc3650 bc3651 bc3652
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3653 bc3654 bc3655 bc3656 bc3657 bc3658 bc3659 bc3660 bc3661 bc3662 bc3663
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3664 bc3665 bc3666 bc3667 bc3668 bc3669 bc3670 bc3671 bc3672 bc3673 bc3674
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3675 bc3676 bc3677 bc3678 bc3679 bc3680 bc3681 bc3682 bc3683 bc3684 bc3685
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3686 bc3687 bc3688 bc3689 bc3690 bc3691 bc3692 bc3693 bc3694 bc3695 bc3696
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3697 bc3698 bc3699 bc3700 bc3701 bc3702 bc3703 bc3704 bc3705 bc3706 bc3707
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3708 bc3709 bc3710 bc3711 bc3712 bc3713 bc3714 bc3715 bc3716 bc3717 bc3718
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3719 bc3720 bc3721 bc3722 bc3723 bc3724 bc3725 bc3726 bc3727 bc3728 bc3729
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3730 bc3731 bc3732 bc3733 bc3734 bc3735 bc3736 bc3737 bc3738 bc3739 bc3740
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3741 bc3742 bc3743 bc3744 bc3745 bc3746 bc3747 bc3748 bc3749 bc3750 bc3751
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3752 bc3753 bc3754 bc3755 bc3756 bc3757 bc3758 bc3759 bc3760 bc3761 bc3762
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3763 bc3764 bc3765 bc3766 bc3767 bc3768 bc3769 bc3770 bc3771 bc3772 bc3773
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3774 bc3775 bc3776 bc3777 bc3778 bc3779 bc3780 bc3781 bc3782 bc3783 bc3784
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3785 bc3786 bc3787 bc3788 bc3789 bc3790 bc3791 bc3792 bc3793 bc3794 bc3795
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3796 bc3797 bc3798 bc3799 bc3800 bc3801 bc3802 bc3803 bc3804 bc3805 bc3806
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3807 bc3808 bc3809 bc3810 bc3811 bc3812 bc3813 bc3814 bc3815 bc3816 bc3817
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3818 bc3819 bc3820 bc3821 bc3822 bc3823 bc3824 bc3825 bc3826 bc3827 bc3828
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3829 bc3830 bc3831 bc3832 bc3833 bc3834 bc3835 bc3836 bc3837 bc3838 bc3839
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3840 bc3841 bc3842 bc3843 bc3844 bc3845 bc3846 bc3847 bc3848 bc3849 bc3850
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3851 bc3852 bc3853 bc3854 bc3855 bc3856 bc3857 bc3858 bc3859 bc3860 bc3861
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3862 bc3863 bc3864 bc3865 bc3866 bc3867 bc3868 bc3869 bc3870 bc3871 bc3872
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3873 bc3874 bc3875 bc3876 bc3877 bc3878 bc3879 bc3880 bc3881 bc3882 bc3883
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3884 bc3885 bc3886 bc3887 bc3888 bc3889 bc3890 bc3891 bc3892 bc3893 bc3894
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3895 bc3896 bc3897 bc3898 bc3899 bc3900 bc3901 bc3902 bc3903 bc3904 bc3905
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3906 bc3907 bc3908 bc3909 bc3910 bc3911 bc3912 bc3913 bc3914 bc3915 bc3916
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3917 bc3918 bc3919 bc3920 bc3921 bc3922 bc3923 bc3924 bc3925 bc3926 bc3927
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3928 bc3929 bc3930 bc3931 bc3932 bc3933 bc3934 bc3935 bc3936 bc3937 bc3938
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3939 bc3940 bc3941 bc3942 bc3943 bc3944 bc3945 bc3946 bc3947 bc3948 bc3949
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3950 bc3951 bc3952 bc3953 bc3954 bc3955 bc3956 bc3957 bc3958 bc3959 bc3960
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3961 bc3962 bc3963 bc3964 bc3965 bc3966 bc3967 bc3968 bc3969 bc3970 bc3971
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3972 bc3973 bc3974 bc3975 bc3976 bc3977 bc3978 bc3979 bc3980 bc3981 bc3982
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3983 bc3984 bc3985 bc3986 bc3987 bc3988 bc3989 bc3990 bc3991 bc3992 bc3993
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc3994 bc3995 bc3996 bc3997 bc3998 bc3999 bc4000 bc4001 bc4002 bc4003 bc4004
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4005 bc4006 bc4007 bc4008 bc4009 bc4010 bc4011 bc4012 bc4013 bc4014 bc4015
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4016 bc4017 bc4018 bc4019 bc4020 bc4021 bc4022 bc4023 bc4024 bc4025 bc4026
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4027 bc4028 bc4029 bc4030 bc4031 bc4032 bc4033 bc4034 bc4035 bc4036 bc4037
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4038 bc4039 bc4040 bc4041 bc4042 bc4043 bc4044 bc4045 bc4046 bc4047 bc4048
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4049 bc4050 bc4051 bc4052 bc4053 bc4054 bc4055 bc4056 bc4057 bc4058 bc4059
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4060 bc4061 bc4062 bc4063 bc4064 bc4065 bc4066 bc4067 bc4068 bc4069 bc4070
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4071 bc4072 bc4073 bc4074 bc4075 bc4076 bc4077 bc4078 bc4079 bc4080 bc4081
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4082 bc4083 bc4084 bc4085 bc4086 bc4087 bc4088 bc4089 bc4090 bc4091 bc4092
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4093 bc4094 bc4095 bc4096 bc4097 bc4098 bc4099 bc4100 bc4101 bc4102 bc4103
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4104 bc4105 bc4106 bc4107 bc4108 bc4109 bc4110 bc4111 bc4112 bc4113 bc4114
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4115 bc4116 bc4117 bc4118 bc4119 bc4120 bc4121 bc4122 bc4123 bc4124 bc4125
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4126 bc4127 bc4128 bc4129 bc4130 bc4131 bc4132 bc4133 bc4134 bc4135 bc4136
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4137 bc4138 bc4139 bc4140 bc4141 bc4142 bc4143 bc4144 bc4145 bc4146 bc4147
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4148 bc4149 bc4150 bc4151 bc4152 bc4153 bc4154 bc4155 bc4156 bc4157 bc4158
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4159 bc4160 bc4161 bc4162 bc4163 bc4164 bc4165 bc4166 bc4167 bc4168 bc4169
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4170 bc4171 bc4172 bc4173 bc4174 bc4175 bc4176 bc4177 bc4178 bc4179 bc4180
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4181 bc4182 bc4183 bc4184 bc4185 bc4186 bc4187 bc4188 bc4189 bc4190 bc4191
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4192 bc4193 bc4194 bc4195 bc4196 bc4197 bc4198 bc4199 bc4200 bc4201 bc4202
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4203 bc4204 bc4205 bc4206 bc4207 bc4208 bc4209 bc4210 bc4211 bc4212 bc4213
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4214 bc4215 bc4216 bc4217 bc4218 bc4219 bc4220 bc4221 bc4222 bc4223 bc4224
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4225 bc4226 bc4227 bc4228 bc4229 bc4230 bc4231 bc4232 bc4233 bc4234 bc4235
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4236 bc4237 bc4238 bc4239 bc4240 bc4241 bc4242 bc4243 bc4244 bc4245 bc4246
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4247 bc4248 bc4249 bc4250 bc4251 bc4252 bc4253 bc4254 bc4255 bc4256 bc4257
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4258 bc4259 bc4260 bc4261 bc4262 bc4263 bc4264 bc4265 bc4266 bc4267 bc4268
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4269 bc4270 bc4271 bc4272 bc4273 bc4274 bc4275 bc4276 bc4277 bc4278 bc4279
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4280 bc4281 bc4282 bc4283 bc4284 bc4285 bc4286 bc4287 bc4288 bc4289 bc4290
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4291 bc4292 bc4293 bc4294 bc4295 bc4296 bc4297 bc4298 bc4299 bc4300 bc4301
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4302 bc4303 bc4304 bc4305 bc4306 bc4307 bc4308 bc4309 bc4310 bc4311 bc4312
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4313 bc4314 bc4315 bc4316 bc4317 bc4318 bc4319 bc4320 bc4321 bc4322 bc4323
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4324 bc4325 bc4326 bc4327 bc4328 bc4329 bc4330 bc4331 bc4332 bc4333 bc4334
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4335 bc4336 bc4337 bc4338 bc4339 bc4340 bc4341 bc4342 bc4343 bc4344 bc4345
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4346 bc4347 bc4348 bc4349 bc4350 bc4351 bc4352 bc4353 bc4354 bc4355 bc4356
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4357 bc4358 bc4359 bc4360 bc4361 bc4362 bc4363 bc4364 bc4365 bc4366 bc4367
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4368 bc4369 bc4370 bc4371 bc4372 bc4373 bc4374 bc4375 bc4376 bc4377 bc4378
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4379 bc4380 bc4381 bc4382 bc4383 bc4384 bc4385 bc4386 bc4387 bc4388 bc4389
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4390 bc4391 bc4392 bc4393 bc4394 bc4395 bc4396 bc4397 bc4398 bc4399 bc4400
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4401 bc4402 bc4403 bc4404 bc4405 bc4406 bc4407 bc4408 bc4409 bc4410 bc4411
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4412 bc4413 bc4414 bc4415 bc4416 bc4417 bc4418 bc4419 bc4420 bc4421 bc4422
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4423 bc4424 bc4425 bc4426 bc4427 bc4428 bc4429 bc4430 bc4431 bc4432 bc4433
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4434 bc4435 bc4436 bc4437 bc4438 bc4439 bc4440 bc4441 bc4442 bc4443 bc4444
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4445 bc4446 bc4447 bc4448 bc4449 bc4450 bc4451 bc4452 bc4453 bc4454 bc4455
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4456 bc4457 bc4458 bc4459 bc4460 bc4461 bc4462 bc4463 bc4464 bc4465 bc4466
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4467 bc4468 bc4469 bc4470 bc4471 bc4472 bc4473 bc4474 bc4475 bc4476 bc4477
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4478 bc4479 bc4480 bc4481 bc4482 bc4483 bc4484 bc4485 bc4486 bc4487 bc4488
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4489 bc4490 bc4491 bc4492 bc4493 bc4494 bc4495 bc4496 bc4497 bc4498 bc4499
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4500 bc4501 bc4502 bc4503 bc4504 bc4505 bc4506 bc4507 bc4508 bc4509 bc4510
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4511 bc4512 bc4513 bc4514 bc4515 bc4516 bc4517 bc4518 bc4519 bc4520 bc4521
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4522 bc4523 bc4524 bc4525 bc4526 bc4527 bc4528 bc4529 bc4530 bc4531 bc4532
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4533 bc4534 bc4535 bc4536 bc4537 bc4538 bc4539 bc4540 bc4541 bc4542 bc4543
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4544 bc4545 bc4546 bc4547 bc4548 bc4549 bc4550 bc4551 bc4552 bc4553 bc4554
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4555 bc4556 bc4557 bc4558 bc4559 bc4560 bc4561 bc4562 bc4563 bc4564 bc4565
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4566 bc4567 bc4568 bc4569 bc4570 bc4571 bc4572 bc4573 bc4574 bc4575 bc4576
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4577 bc4578 bc4579 bc4580 bc4581 bc4582 bc4583 bc4584 bc4585 bc4586 bc4587
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4588 bc4589 bc4590 bc4591 bc4592 bc4593 bc4594 bc4595 bc4596 bc4597 bc4598
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4599 bc4600 bc4601 bc4602 bc4603 bc4604 bc4605 bc4606 bc4607 bc4608 bc4609
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4610 bc4611 bc4612 bc4613 bc4614 bc4615 bc4616 bc4617 bc4618 bc4619 bc4620
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4621 bc4622 bc4623 bc4624 bc4625 bc4626 bc4627 bc4628 bc4629 bc4630 bc4631
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4632 bc4633 bc4634 bc4635 bc4636 bc4637 bc4638 bc4639 bc4640 bc4641 bc4642
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4643 bc4644 bc4645 bc4646 bc4647 bc4648 bc4649 bc4650 bc4651 bc4652 bc4653
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4654 bc4655 bc4656 bc4657 bc4658 bc4659 bc4660 bc4661 bc4662 bc4663 bc4664
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4665 bc4666 bc4667 bc4668 bc4669 bc4670 bc4671 bc4672 bc4673 bc4674 bc4675
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4676 bc4677 bc4678 bc4679 bc4680 bc4681 bc4682 bc4683 bc4684 bc4685 bc4686
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4687 bc4688 bc4689 bc4690 bc4691 bc4692 bc4693 bc4694 bc4695 bc4696 bc4697
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4698 bc4699 bc4700 bc4701 bc4702 bc4703 bc4704 bc4705 bc4706 bc4707 bc4708
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4709 bc4710 bc4711 bc4712 bc4713 bc4714 bc4715 bc4716 bc4717 bc4718 bc4719
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4720 bc4721 bc4722 bc4723 bc4724 bc4725 bc4726 bc4727 bc4728 bc4729 bc4730
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4731 bc4732 bc4733 bc4734 bc4735 bc4736 bc4737 bc4738 bc4739 bc4740 bc4741
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4742 bc4743 bc4744 bc4745 bc4746 bc4747 bc4748 bc4749 bc4750 bc4751 bc4752
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4753 bc4754 bc4755 bc4756 bc4757 bc4758 bc4759 bc4760 bc4761 bc4762 bc4763
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4764 bc4765 bc4766 bc4767 bc4768 bc4769 bc4770 bc4771 bc4772 bc4773 bc4774
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4775 bc4776 bc4777 bc4778 bc4779 bc4780 bc4781 bc4782 bc4783 bc4784 bc4785
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4786 bc4787 bc4788 bc4789 bc4790 bc4791 bc4792 bc4793 bc4794 bc4795 bc4796
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4797 bc4798 bc4799 bc4800 bc4801 bc4802 bc4803 bc4804 bc4805 bc4806 bc4807
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4808 bc4809 bc4810 bc4811 bc4812 bc4813 bc4814 bc4815 bc4816 bc4817 bc4818
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4819 bc4820 bc4821 bc4822 bc4823 bc4824 bc4825 bc4826 bc4827 bc4828 bc4829
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4830 bc4831 bc4832 bc4833 bc4834 bc4835 bc4836 bc4837 bc4838 bc4839 bc4840
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4841 bc4842 bc4843 bc4844 bc4845 bc4846 bc4847 bc4848 bc4849 bc4850 bc4851
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4852 bc4853 bc4854 bc4855 bc4856 bc4857 bc4858 bc4859 bc4860 bc4861 bc4862
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4863 bc4864 bc4865 bc4866 bc4867 bc4868 bc4869 bc4870 bc4871 bc4872 bc4873
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4874 bc4875 bc4876 bc4877 bc4878 bc4879 bc4880 bc4881 bc4882 bc4883 bc4884
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4885 bc4886 bc4887 bc4888 bc4889 bc4890 bc4891 bc4892 bc4893 bc4894 bc4895
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4896 bc4897 bc4898 bc4899 bc4900 bc4901 bc4902 bc4903 bc4904 bc4905 bc4906
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4907 bc4908 bc4909 bc4910 bc4911 bc4912 bc4913 bc4914 bc4915 bc4916 bc4917
## YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT YuT
## bc4918 bc4919 bc4920 bc4921 bc4922
## YuT YuT YuT YuT YuT
## Levels: YuTYuT$orig.ident <- "YuT"#Plotting Yu
YuC[["percent.mt"]] <- PercentageFeatureSet(YuC, pattern = "^mt-")
YuT[["percent.mt"]] <- PercentageFeatureSet(YuT, pattern = "^mt-")
VlnPlot(YuC, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
VlnPlot(YuT, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
##Filtering Yu 2000 < nFeature_RNA
5,000 < nCount_RNA <100,000
0 =< percent.mt < 10
YuC.filtered <- subset(YuC, subset = nFeature_RNA > 2000 & nCount_RNA > 5000 & nCount_RNA < 100000 & percent.mt < 10)
VlnPlot(YuC.filtered, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt" ), ncol = 3, log=TRUE)
YuT.filtered <- subset(YuT, subset = nFeature_RNA > 2000 & nCount_RNA > 5000 & nCount_RNA < 100000 & percent.mt < 10)
VlnPlot(YuT.filtered, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt" ), ncol = 3, log=TRUE)
#Shen
ShenNC<-read.delim(file = "/home/moyra.lawrence/R_analysis/Comparison/Shen/GSM5195022_SC-siNC-count.csv.gz", header = TRUE, sep = ",")
ShenSnrpd2<-read.delim(file = "/home/moyra.lawrence/R_analysis/Comparison/Shen/GSM5195023_SC-siSnrpd2-count.csv.gz", header = TRUE, sep = ",")
ShenPSC<-read.delim(file = "/home/moyra.lawrence/R_analysis/Comparison/Shen/GSM5195024_SC-PSC-count.csv.gz", header = TRUE, sep = ",")
ShenTBLC<-read.delim(file = "/home/moyra.lawrence/R_analysis/Comparison/Shen/GSM5195025_SC-TBLC-count.csv.gz", header = TRUE, sep = ",")
ShenNCS <- CreateSeuratObject(counts = ShenNC, project = "ShenNC", min.cells = 3, min.features = 200)
ShenSnrpd2S <- CreateSeuratObject(counts = ShenSnrpd2, project = "ShenSnrpd2", min.cells = 3, min.features = 200)
ShenPSCS <- CreateSeuratObject(counts = ShenPSC, project = "ShenPSC", min.cells = 3, min.features = 200)
ShenTBLCS <- CreateSeuratObject(counts = ShenTBLC, project = "ShenTBLC", min.cells = 3, min.features = 200)#Plotting Shen
ShenNCS[["percent.mt"]] <- PercentageFeatureSet(ShenNCS, pattern = "^mt-")
ShenSnrpd2S[["percent.mt"]] <- PercentageFeatureSet(ShenSnrpd2S, pattern = "^mt-")
ShenPSCS[["percent.mt"]] <- PercentageFeatureSet(ShenPSCS, pattern = "^mt-")
ShenTBLCS[["percent.mt"]] <- PercentageFeatureSet(ShenTBLCS, pattern = "^mt-")
VlnPlot(ShenNCS, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
VlnPlot(ShenSnrpd2S, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
VlnPlot(ShenPSCS, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
VlnPlot(ShenTBLCS, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3, log=TRUE)
Loading embryo data and preparing for seurat
raw_data <- data.table::fread("/home/moyra.lawrence/R_analysis/Second_10x_run/GSE45719_matrix.txt.gz", data.table = FALSE)
names(raw_data)[1] <- 'Gene'
names <- make.unique(raw_data$Gene)
rownames(raw_data) <- names
raw_data <- raw_data[,-1] # get rid of old names
colnames(raw_data) <- gsub("_", "-", colnames(raw_data))
colnames(raw_data) <- stri_replace_all_fixed(str = colnames(raw_data), pattern = "_", replacement = "-")
colnames(raw_data) <- stri_replace_all_fixed(str = colnames(raw_data), pattern = "zy1", replacement = "zygote-1")
colnames(raw_data) <- stri_replace_all_fixed(str = colnames(raw_data), pattern = "zy2", replacement = "zygote-2")
colnames(raw_data) <- stri_replace_all_fixed(str = colnames(raw_data), pattern = "zy3", replacement = "zygote-3")
colnames(raw_data) <- stri_replace_all_fixed(str = colnames(raw_data), pattern = "zy4", replacement = "zygote-4")
ML11 <- CreateSeuratObject(counts=raw_data, project="ML11", names.delim="-")## Warning: Feature names cannot have pipe characters ('|'), replacing with dashes
## ('-')ML11$orig.ident## 16cell-1-10 16cell-1-11 16cell-1-12
## 16cell 16cell 16cell
## 16cell-1-13 16cell-1-14 16cell-1-15
## 16cell 16cell 16cell
## 16cell-1-2 16cell-1-3 16cell-1-4
## 16cell 16cell 16cell
## 16cell-1-5 16cell-1-6 16cell-1-7
## 16cell 16cell 16cell
## 16cell-1-8 16cell-1-9 16cell-4-1
## 16cell 16cell 16cell
## 16cell-4-10 16cell-4-11 16cell-4-2
## 16cell 16cell 16cell
## 16cell-4-3 16cell-4-4 16cell-4-5
## 16cell 16cell 16cell
## 16cell-4-6 16cell-4-7 16cell-4-8
## 16cell 16cell 16cell
## 16cell-4-9 16cell-5-1 16cell-5-10
## 16cell 16cell 16cell
## 16cell-5-11 16cell-5-12 16cell-5-13
## 16cell 16cell 16cell
## 16cell-5-2 16cell-5-3 16cell-5-4
## 16cell 16cell 16cell
## 16cell-5-5 16cell-5-6 16cell-5-7
## 16cell 16cell 16cell
## 16cell-5-8 16cell-5-9 16cell-6-1
## 16cell 16cell 16cell
## 16cell-6-10 16cell-6-11 16cell-6-12
## 16cell 16cell 16cell
## 16cell-6-2 16cell-6-3 16cell-6-4
## 16cell 16cell 16cell
## 16cell-6-5 16cell-6-6 16cell-6-7
## 16cell 16cell 16cell
## 16cell-6-8 16cell-6-9 4cell-1-1
## 16cell 16cell 4cell
## 4cell-1-2 4cell-1-4 4cell-2-1
## 4cell 4cell 4cell
## 4cell-2-2 4cell-2-3 4cell-2-4
## 4cell 4cell 4cell
## 4cell-3-1 4cell-3-3 4cell-3-4
## 4cell 4cell 4cell
## 4cell-4-1 4cell-4-2 4cell-4-3
## 4cell 4cell 4cell
## 4cell-4-4 8cell-1-1 8cell-1-2
## 4cell 8cell 8cell
## 8cell-1-4 8cell-1-5 8cell-1-6
## 8cell 8cell 8cell
## 8cell-1-7 8cell-1-8 8cell-2-1
## 8cell 8cell 8cell
## 8cell-2-2 8cell-2-3 8cell-2-4
## 8cell 8cell 8cell
## 8cell-2-6 8cell-2-7 8cell-2-8
## 8cell 8cell 8cell
## 8cell-5-1 8cell-5-2 8cell-5-3
## 8cell 8cell 8cell
## 8cell-5-4 8cell-5-6 8cell-5-7
## 8cell 8cell 8cell
## 8cell-5-8 8cell-8-1 8cell-8-2
## 8cell 8cell 8cell
## 8cell-8-3 8cell-8-4 8cell-8-6
## 8cell 8cell 8cell
## 8cell-8-7 8cell-8-8 BXC-100pg-liver-RNA-1
## 8cell 8cell BXC
## BXC-100pg-liver-RNA-2 BXC-10pg-liver-RNA-1 BXC-10pg-liver-RNA-2
## BXC BXC BXC
## BXC-1ng-liver-RNA-0r BXC-1ng-liver-RNA-1 BXC-30pg-liver-RNA-0r
## BXC BXC BXC
## BXC-30pg-liver-RNA-2 BXC-liver-cell-1 BXC-liver-cell-2
## BXC BXC BXC
## BXC-liver-cell-4 BXC-liver-cell-5 BXC-liver-cell-6
## BXC BXC BXC
## C57twocell-16-1 C57twocell-16-2 C57twocell-18-1
## C57twocell C57twocell C57twocell
## C57twocell-18-2 C57twocell-20-1 C57twocell-20-2
## C57twocell C57twocell C57twocell
## C57twocell-22-1 C57twocell-22-2 early2cell-0r-1
## C57twocell C57twocell early2cell
## early2cell-0r-2 early2cell-1-1 early2cell-1-2
## early2cell early2cell early2cell
## early2cell-2-1 early2cell-2-2 early2cell-3-1
## early2cell early2cell early2cell
## early2cell-3-2 earlyblast-2-1 earlyblast-2-10
## early2cell earlyblast earlyblast
## earlyblast-2-12 earlyblast-2-15 earlyblast-2-16
## earlyblast earlyblast earlyblast
## earlyblast-2-17 earlyblast-2-19 earlyblast-2-2
## earlyblast earlyblast earlyblast
## earlyblast-2-22 earlyblast-2-3 earlyblast-2-4
## earlyblast earlyblast earlyblast
## earlyblast-2-5 earlyblast-2-6 earlyblast-2-7
## earlyblast earlyblast earlyblast
## earlyblast-2-8 earlyblast-3-1 earlyblast-3-10
## earlyblast earlyblast earlyblast
## earlyblast-3-12 earlyblast-3-13 earlyblast-3-14
## earlyblast earlyblast earlyblast
## earlyblast-3-15 earlyblast-3-16 earlyblast-3-17
## earlyblast earlyblast earlyblast
## earlyblast-3-2 earlyblast-3-3 earlyblast-3-4
## earlyblast earlyblast earlyblast
## earlyblast-3-6 earlyblast-3-7 earlyblast-3-8
## earlyblast earlyblast earlyblast
## earlyblast-3-9 earlyblast-4-1 earlyblast-4-12
## earlyblast earlyblast earlyblast
## earlyblast-4-13 earlyblast-4-14 earlyblast-4-16
## earlyblast earlyblast earlyblast
## earlyblast-4-17 earlyblast-4-18 earlyblast-4-2
## earlyblast earlyblast earlyblast
## earlyblast-4-3 earlyblast-4-5 earlyblast-4-6
## earlyblast earlyblast earlyblast
## earlyblast-4-8 earlyblast-4-9 late2cell-5-1
## earlyblast earlyblast late2cell
## late2cell-5-2 late2cell-6-1 late2cell-6-2
## late2cell late2cell late2cell
## late2cell-7-1 late2cell-7-2 late2cell-8-1
## late2cell late2cell late2cell
## late2cell-8-2 late2cell-9-1 late2cell-9-2
## late2cell late2cell late2cell
## lateblast-1-10 lateblast-1-11 lateblast-1-13
## lateblast lateblast lateblast
## lateblast-1-14 lateblast-1-16 lateblast-1-19
## lateblast lateblast lateblast
## lateblast-1-2 lateblast-1-20 lateblast-1-21
## lateblast lateblast lateblast
## lateblast-1-23 lateblast-1-24 lateblast-1-26
## lateblast lateblast lateblast
## lateblast-1-27 lateblast-1-4 lateblast-1-5
## lateblast lateblast lateblast
## lateblast-1-6 lateblast-1-7 lateblast-1-8
## lateblast lateblast lateblast
## lateblast-1-9 lateblast-2-1 lateblast-2-12
## lateblast lateblast lateblast
## lateblast-2-14 lateblast-2-16 lateblast-2-17
## lateblast lateblast lateblast
## lateblast-2-2 lateblast-2-3 lateblast-2-5
## lateblast lateblast lateblast
## lateblast-2-7 lateblast-2-8 lateblast-2-9
## lateblast lateblast lateblast
## mid2cell-0r-1 mid2cell-0r-2 mid2cell-3-1
## mid2cell mid2cell mid2cell
## mid2cell-3-2 mid2cell-4-1 mid2cell-4-2
## mid2cell mid2cell mid2cell
## mid2cell-5-1 mid2cell-5-2 mid2cell-6-1
## mid2cell mid2cell mid2cell
## mid2cell-6-2 mid2cell-7-1 mid2cell-7-2
## mid2cell mid2cell mid2cell
## midblast-1-1 midblast-1-10 midblast-1-11
## midblast midblast midblast
## midblast-1-12 midblast-1-13 midblast-1-14
## midblast midblast midblast
## midblast-1-15 midblast-1-16 midblast-1-17
## midblast midblast midblast
## midblast-1-18 midblast-1-19 midblast-1-2
## midblast midblast midblast
## midblast-1-20 midblast-1-22 midblast-1-23
## midblast midblast midblast
## midblast-1-24 midblast-1-3 midblast-1-4
## midblast midblast midblast
## midblast-1-5 midblast-1-6 midblast-1-8
## midblast midblast midblast
## midblast-1-9 midblast-2-1 midblast-2-10
## midblast midblast midblast
## midblast-2-11 midblast-2-12 midblast-2-13
## midblast midblast midblast
## midblast-2-14 midblast-2-15 midblast-2-16
## midblast midblast midblast
## midblast-2-17 midblast-2-18 midblast-2-2
## midblast midblast midblast
## midblast-2-23 midblast-2-24 midblast-2-3
## midblast midblast midblast
## midblast-2-4 midblast-2-5 midblast-2-6
## midblast midblast midblast
## midblast-2-7 midblast-2-8 midblast-2-9
## midblast midblast midblast
## midblast-3-1 midblast-3-10 midblast-3-11
## midblast midblast midblast
## midblast-3-12 midblast-3-13 midblast-3-14
## midblast midblast midblast
## midblast-3-15 midblast-3-17 midblast-3-18
## midblast midblast midblast
## midblast-3-2 midblast-3-23 midblast-3-3
## midblast midblast midblast
## midblast-3-4 midblast-3-5 midblast-3-6
## midblast midblast midblast
## midblast-3-7 midblast-3-8 midblast-3-9
## midblast midblast midblast
## zygote-1 zygote-2 zygote-3
## zygote zygote zygote
## zygote-4 16cell-2pooled-split6a 16cell-2pooled-split6b
## zygote 16cell 16cell
## 16cell-2pooled-split8a 16cell-2pooled-split8b 16cell-split1a
## 16cell 16cell 16cell
## 16cell-split1b 16cell-split2a 16cell-split2b
## 16cell 16cell 16cell
## 8cell-12-1-smartseq2 8cell-12-2-smartseq2 8cell-12-3-smartseq2
## 8cell 8cell 8cell
## 8cell-12-4-smartseq2 8cell-13-1-smartseq2 8cell-14-1-smartseq2
## 8cell 8cell 8cell
## 8cell-14-2-smartseq2 8cell-14-3-smartseq2 8cell-14-4-smartseq2
## 8cell 8cell 8cell
## 8cell-2pooled-split5a 8cell-2pooled-split5b 8cell-2pooled-split7a
## 8cell 8cell 8cell
## 8cell-2pooled-split7b 8cell-2pooled-split9a 8cell-2pooled-split9b
## 8cell 8cell 8cell
## 8cell-split3a 8cell-split3b 8cell-split4a
## 8cell 8cell 8cell
## 8cell-split4b fibroblast-13-CxB fibroblast-14-CxB
## 8cell fibroblast fibroblast
## fibroblast-15-CxB fibroblast-16-CxB fibroblast-17-BxC
## fibroblast fibroblast fibroblast
## fibroblast-19-BxC fibroblast-20-BxC fibroblast-21-BxC
## fibroblast fibroblast fibroblast
## fibroblast-22-BxC fibroblast-9-CxB
## fibroblast fibroblast
## 13 Levels: 16cell 4cell 8cell BXC C57twocell early2cell ... zygoteML11[["in.vivo"]] <- Idents(object = ML11)
ML11$orig.ident <- "ML11"Loading data
ML12 <- read_rds(file='/home/moyra.lawrence/count3/enumerate/EVEV/seurat.obj.rds')
ML13 <- read_rds(file='/home/moyra.lawrence/count3/enumerate/Dppa2_MERVL_TT/seurat.obj.rds')
ML12## An object of class Seurat
## 32294 features across 3438 samples within 1 assay
## Active assay: RNA (32294 features, 0 variable features)ML13## An object of class Seurat
## 32294 features across 3958 samples within 1 assay
## Active assay: RNA (32294 features, 0 variable features)##Filtering
ML12[["percent.mt"]] <- PercentageFeatureSet(ML12, pattern = "^mt-")
ML13[["percent.mt"]] <- PercentageFeatureSet(ML13, pattern = "^mt-")Filtering conditions for EVEV
3500 < nFeature_RNA
10,000 < nCount_RNA <120,000
0 =< percent.mt < 10
Filtering conditions for DMTT
2000 < nFeature_RNA
10,000 < nCount_RNA <120,000
0 =< percent.mt < 15
Filtering datasets
ML12.filtered <- subset(ML12, subset = nFeature_RNA > 3500 & nCount_RNA > 10000 & nCount_RNA < 120000 & percent.mt < 10)
ML13.filtered <- subset(ML13, subset = nFeature_RNA > 2000 & nCount_RNA > 10000 & nCount_RNA < 120000 & percent.mt < 15)
VlnPlot(ML12.filtered, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt" ), ncol = 3, log=TRUE)
VlnPlot(ML13.filtered, pt.size=0, features = c("nFeature_RNA", "nCount_RNA", "percent.mt" ), ncol = 3, log=TRUE)
#Fixing orig.ident
Hu.filtered$orig.ident <- "Hu"
XuESC1.filtered$orig.ident <- "XuESC1"
XuESC2.filtered$orig.ident <- "XuESC2"
XuEPS1.filtered$orig.ident <- "XuEPS1"
XuEPS2.filtered$orig.ident <- "XuEPS2"
XuEPSTPS.filtered$orig.ident <- "XuEPSTPS"
Xu2CTPS.filtered$orig.ident <- "Xu2CTPS"
ShenNCS$orig.ident <- "ShenNCS"
ShenSnrpd2S$orig.ident <- "ShenSnrpd2S"
ShenPSCS$orig.ident <- "ShenPSCS"
ShenTBLCS$orig.ident <- "ShenTBLCS"Merging data
merge.filtered <- merge(ML12.filtered, y = c(ML11, ML13.filtered, Hu.filtered, XuESC1.filtered, XuESC2.filtered, XuEPS1.filtered, XuEPS2.filtered, XuEPSTPS.filtered, Xu2CTPS.filtered, Cossec.filtered, YuC.filtered, YuT.filtered, ShenNCS, ShenSnrpd2S, ShenPSCS, ShenTBLCS), add.cell.ids = c("Empty_puro_Empty_hygro", "Embryo", "Dppa2_MERVL_TT", "Hu", "XuESC1", "XuESC2", "XuEPS1", "XuEPS2", "XuEPSTPS", "Xu2CTPS", "Cossec", "Yu_Control", "Yu_CX5461", "ShenNC", "ShenSnrpd2", "ShenPSC", "ShenTBLC"), project = "ML1")
merge.filtered## An object of class Seurat
## 59223 features across 92170 samples within 1 assay
## Active assay: RNA (59223 features, 0 variable features)save(merge.filtered, file="merge_filtered3.rda")
#load(file='merge_filtered3.rda')